Serological Diagnosis of Camel Brucellosis at Sharkia Governorate, Egypt

Document Type : Original Article

Authors

Abstract

The present study was carried out on 210 camels serum samples collected from a various abattoirs at Sharkia Governorate to clarify the prevalence of the brucellosis among camels during the period from June 2012 to August 2013 in addition to find the most reliable diagnostic method. In this investigation, serological tests on 210 camel sera using Buffered Acidified Plate Antigen Test (BAPAT), Rose Bengal Plate Test (RBPT), Rivanol Test (Riv.T), Complement Fixation Test (CFT) and Immunochromatographic Assay (ICA) to evaluate  traditional and recent tests which used for the diagnosis.                                                                                                         
 The obtained results were 7.6%, 6.7%, 6.2%, 6.2% and 6.7%  using BAPAT, RBPT, Riv.T, CFT and ICA  respectively .The sensitivity percent of different serological tests were 95.45%, 87.75%, 90.90%, 8885% and 92.15% for BAPAT, RBPT, Riv. T., CFT and ICA respectively. While their specificity percent were 99.27%, 99.61%, 99.80%, 99.80% and 99.75% for the same test respectively.                                                           
ICA could be recommended as a rapid screening test, easily performed, sensitive and high specific and as confirmatory test for diagnosis of brucellosis in camels. So, ICA is the method of choice when testing animals in remote areas, nomadic and other migratory population. Thus study throw a spot light to include camels in the national program for control and eradication of brucellosis in Egypt on the base that where brucellosis exists in stock animals.
 

Main Subjects