Document Type : Original Article
Authors
1
Artifcial Insemination and Embryo Transfer Dept., Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
2
Immunology and Immunopharmacology Unit , Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
3
Dept. of Field Investigations, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
Abstract
The present study aimed to investigate the cryoprotective capacities of natural and lyophilized forms of whole egg yolk, egg plasma and low-density lipoprotein (LDL) on Arab stallion spermatozoa. Semen samples were collected from 5 Arabian stallions and diluted with INRA-82 extender containing natural egg yolk (15%), lyophilized egg yolk (15%), natural egg plasma (5, 7.5, 10 and 15%), lypholized egg plasma (10%), natural LDL (5, 7.5, 10 and 15%) and lypholized LDL (7.5%). Semen aliquots were processesed for cryopreservation and sperm pos-thaw motility, membrane; acrosome, mitochondria and DNA integrities were recorded. The results showed that, 10% egg plasma and 7.5% LDL were superior to other tested concentrations. Natural forms of LDL and egg plasma improved quality of frozen-thawed stallion spermatozoa in terms of pos-thaw motility, viability index, membrane, acrosome, mitochondria and DNA integrities. Using the lyophilized forms of LDL and egg plasma in stallion semen extender resulted in the worst semen quality endpoints, while, using the lyophilized egg yolk resulted in satisfactory results. In conclusion, the addition of the natural forms of LDL and egg plasma as well as lyophilized egg yolk to semen extender could be good alternatives to the whole egg yolk as they improves stallion sperm quality after cryopreservation in terms of motility, integrities of mitochondria, membrane and acrosome as well as reduction of DNA damage.
Main Subjects
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