Document Type : Original Article
Authors
1
Bacteriology, Biochemistry Departments, Animal Health Research Institute (Zagazig Branch)
2
Clinical Pathology Department, Animal Health Research Institute (Zagazig ranch)
3
Clinical Pathology Department, Animal Health Research Institute (Zagazig Branch)
Abstract
Sixty broiler chickens of one day old were used to evaluate the effects of humic acid on body performance, leukogram and some biochemical parameters as well as effect in clostridium preferences (C. perferenges). At 20 of age 30 chicks were experementally infected with C. perferenges .Boiler chickens were divided into 4 equal groups (15 chicks each), 1st group healthy broiler chickens kept as control group, 2nd group healthy broiler chickens supplemented with 20 mg/kgm Bwt humic acid in drinking water for 5 successive days, 3rd group broiler chickens experimentally infected with C. perferenges non treated and 4th group broiler chickens experimentally infected with C. perferenges supplemented with 20 mg/kgm Bwt humic acid in drinking water for 5 successive day. Five chicks from each group were sacrificed at 1st and 10thday post supplementation. Two blood samples were taken for hematological biochemical study. Broiler chicken infected with C. perferenges showed clinical signs as loss of appetite, drooping wings, ruffled feathers, depression, emaciation, diarrhea, mortality rate 33.33 %, significant decreases in weight gain, heterophile, phagocytosis, phagocytic index, Killing %, total protein, albumin, A/G ratio beside insignificant eosinophilia and basophilia coupled with significant elevation in feed conversion rate, leukocytic count, lymphocyte, monocyte, total, γ and α globulins, aspartate aminotransferase, alanine aminotransferase, Alkaline phosphates, uric acid and creatinine. Healthy broiler chickens supplemented with humic acid showed significant increases in weight gains, leukocytic count, lymphocyte, phagocytosis, phagocytic index, killing %, total proteins, a , b and g globulin, coupled with significant decreases in concentration of C perfringes in intestinal content, feed conversion rate, heterophils, monocyte, aspartate aminotransferase, alanine aminotransferase, Alkaline phosphates, A/G ratio beside insignificant decrease of serum total protein, albumin, uric acid, creatinine and insignificant in eosinophilia and basophilia. Diseased broiler chicken supplemented with humic acid showed disappear clinical signs and improved body performance as well as hemato-biochemical parameters. It could be concluded that humic acid induce reduction in concentration of C. perfringens in intestine beside improvement in weight gain, feed conversion rate, biochemical parameters and stimulate the immunity of broiler chickens.
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