Document Type : Original Article
Authors
1
Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
2
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abstract
Cancer is the most common cause for human death following cardiovascular disorders. The present study was carried out to investigate the efficacy of curcumin and/or chlorambucil on modulating cancer induced by Ehrlich ascitic cells (EAC) in mice. One hundred and twenty five femal mice were equally divided into five groups. Group (1) was the normal control, groups (2-5) were injected intraperitoneally with EAC (1.4× 106/0.2 ml). Group (2) was left without treatment (positive control). While groups (3-5) were treated orally with curcumin (20 mg/kg BW), chlorambucil (0.2mg/kg BW) and curcumin (20 mg/kg BW) plus chlorambucil (0.2mg/kg BW), respectively, after 48 hours from transplantation of Ehrlich cells. Blood samples and specimens from peritoneum, liver and kidney were collected from 15 mice in each group at the tenth day of the experiment. Survival analysis was carried out on the remaining animals. The obtained results revealed a significant decrease in body weight, ascetic fluids, total number of Ehrlich cells, number of live cells, serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine, blood urea nitrogen, hepatic malondialdehyde and hepatic P53 and Bax gene expression in all treated groups compared with EAC bearing mice. Moreover, the mean survival time, life span, serum levels of total proteins, albumin, albumin globulins ratio, hepaticactivities of antioxidants (SOD and CAT ) and hepatic Bcl-2 gene expression were increased in curcumin and/or chlorambucil treated mice compared with the non treated animals. The histopathological examination showed that the least degree of changes was in the 5th group.In conclusion there is a synergistic good impact for using curamin as an adjuvant in combination with standard chemotherapy such as chlorambucil.
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