Clinicopathological Studies on Effect of Doxorubicin Hydrochloride on Heart of Rats

The present work was performed to study the anticancer effect of Doxorubicin HCl and Propolis and their combination in the treatment of mammary cancer induced by N-methyl-N-nitrosourea beside the ameliorating effect of Propolis against cardiotoxicity of Doxorubicin HCl using the clinicopathological changes.
 One hundred and twenty five female white rats (two months old and 100   gm body weight) were divided into 5 equal groups .Starting of experiment by induction of carcinogen N-methyl-N-nitrosourea (MNU) into gps. 2-5 while rats kept as control in gp.1.After incidence of mammary tumor in gps. 2-5 begin the treatment which continue for 69 days. Rats were given carcinogen only, Doxorubicin HCl, Propolis and combination of   therapeutic dose from Doxorubicin HCl and Propolis in gps. 2,3,4 and 5 ,respectively. Blood samples were collected after 120 days from carcinogen induction, thirty and seventy days post-treatment.
Gp. 3, showed increasing serum CK and LDH activities and cardiac (MDA) assay in addition to decrease cardiac (CAT) and (SOD) activities thirty and seventy days post-treatment. Those changes were appeared in gp. 5  but to lesser degree.
Gp. 4, showed improvement of the previous parameters. Gp. 2 , showed some changes in these parameters.CA 15.3 showed best value in gps. 3 and 5 especially seventy days post-treatment.