Effect of Propolis, Florfenicol, And Their Combination On Catfish (Clariaz Lazera) Experimentally Infected With Aeromonas hydrophila

Document Type : Original Article

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1 Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University

2 Department of Pharmacology, Animal Health Research Institute, Zagazig Branch

Abstract

The aim of the present study was to compare some biochemical and haematological effects of propolis, florfenicol and their combination against experimentally infected Nile catfish (Clariaz Lazera) with Aeromonas hydrophila. One hundred and twenty five Nile catfish were randomly classified into five equal groups each of 25 fish. Fish in group 1 were fed on basal diet (negative control). Fish in group 2 were inoculated intraperitoneally (I/P) with 0.2 ml of 24 hr broth cultures of A. hydrophila (2.5×108 / ml) and kept without medication (positive control). Fish in group 3 were experimentally infected similarly, and were given the basal diet, containing propolis-ethanolic-extract (10 gm /kg diet). Group 4 were experimentally infected similarly, and treated with florfenicol   (10 mg /kg body weight in feed). Group 5 were experimentally infected similarly, and treated with therapeutic dose of propolis plus florfenicol for 10 successive days. Two blood samples were taken from each fish on 1st, 7th and 14th days post treatment. The findings of this study demonstrated that administration of propolis plus florfenicol improved the haematological and biochemical parameters of infected fish with A. hydrophila compared with administration of propolis or florfenicol alone.

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