Beta-lactamases (bls) are implicated seriously in resistance to β - lactam antibiotics. β- lactam resistance related to Sulfa hydral variabl- beta lactamases (SHV- bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained beta-lactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12.It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs.
Attia, A., Hassan, M., & Elwan, S. (2017). Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria. Zagazig Veterinary Journal, 45(1), 55-63. doi: 10.21608/zvjz.2017.7687
MLA
Adel M. Attia; Mohammed N. Hassan; Shymaa E. Elwan. "Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria". Zagazig Veterinary Journal, 45, 1, 2017, 55-63. doi: 10.21608/zvjz.2017.7687
HARVARD
Attia, A., Hassan, M., Elwan, S. (2017). 'Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria', Zagazig Veterinary Journal, 45(1), pp. 55-63. doi: 10.21608/zvjz.2017.7687
VANCOUVER
Attia, A., Hassan, M., Elwan, S. Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria. Zagazig Veterinary Journal, 2017; 45(1): 55-63. doi: 10.21608/zvjz.2017.7687
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