Identification and Validation of FGF10, SP-B and FOXM1 Genes for Regulating Histogenesis of the Fetal New Zealand White Rabbit (Oryctolagus cuniculus) Lungs

Document Type : Original Article

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Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

Abstract

The current work was designed to observe gene expression of Fibroblast Growth Factor 10 (FGF10), Surfactant protein B (SPB), and Forkhead Box M1 (Foxm1) that regulated histogenesis of rabbit's fetus's lungs from the 20th- 29th days prenatally.  During the course of the experiment, the relative expression of these three genes in rabbit's fetus's lungs was detected in the presence of internal reference for normalization, the housekeeping gene GAPDH, by using quantitative reverse transcriptase PCR (qRT- PCR). The histological study of the fetal lungs revealed that from the 20th-22th gestational days; the lung of rabbit fetuses coexisted in pseudoglandular stage and then enter the canalicular stage from the 25th- 26th gestational days. On the 27th day of gestation, the fetal lung developed to the succeeding stage termed the saccular stage. The final stage, the alveolar stage, began to be differentiated at the end of the gestation period. The transcription level of these three genes in rabbit's fetus's lungs was detected In conclusion, the manifestation of FoxM1 mRNA in early rabbit fetus's lungs was higher than that in the lungs of late-term rabbits. Also, lung maturation and the representation of surfactant proteins were in reverse relation with FoxM1 expression in the rabbit's fetuses prenatally.

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