Genomic Characterization of Pigeon Pox Virus in Egypt

Abd El-Samie, A H; Hashem, Mohamed S; Al-Bakry, Mohamad I; A, Manal

doi:10.21608/zvjz.2015.29351

Genomic Characterization of Pigeon Pox Virus in Egypt

Document Type : Original Article

Authors

¹ Department of virology, Faculty of Veterinary Medicine, Zagazig University

² Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo

³ Department of Virology, Faculty of Veterinary Medicine, Zagazig University

10.21608/zvjz.2015.29351

Abstract

In this study skin scrab lesions were collected from pigeons showing symptoms of pigeon pox infection from different localities in Egypt. The prepared tissue suspension for inoculated in chorioallentioc membrane (CAM) of specific pathogenic free (SPF) 11day embryonated chicken eggs. The characteristic pock lesions were observed on CAM at the 2^nd passage and. For genetic characterization of the isolated Pigeon pox virus the Polymerase Chain Reaction (PCR) was done from both crust sample and CAM by amplification of P4B gene using the specific forward and reverse primers. The presence of bands of 570 bp is considered positive results.

Main Subjects