Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Macrolides and Fluoroquinolones Resistance Mechanisms in Campylobacters and their Incidence in Egypt; a Review Article
1
19
EN
Ashraf
A.
Abd El Tawab
Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt
Ahmed
M.
Ammar
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
ahmedammar_2000@yahoo.com
Heba A.
Ahmed
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Ahmed A.
Hefny
Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
ahmed_vet8_2007@yahoo.com
10.21608/zvjz.2019.28652
<em>Campylobacter</em> species are primarily zoonotic pathogens and recognized as a major cause of human illnesses. Poultry, especially, chicken is the main reservoir of <em>Campylobacter </em>species. The uncontrolled use of antibiotics in prophylaxis and treatment of animals caused an increase in antibiotic resistance to macrolides and fluoroquinolones (FQ) which are considered the drugs of choice for treatment of Campylobacter infection. Recently, studies suggested that multidrug efflux systems have the main role in lowering the efficacy of new and old antibiotics. Consequently, efforts are made to find suitable substances to reverse the action of the efflux pumps and prevent antimicrobial resistance. The substances used for evaluation of efflux pumps modulation are either efflux pump inhibitors (EPIs) or efflux pump inducers. The different types of EPIs; phenylalanine arginyl β-naphthylamide, verapamil and phenothiazines, of different mode of actions were used to suppress the activity of different types of efflux pumps. Aspirin, a drug in livestock and poultry, is a nonsteroidal anti-inflammatory, which induces non-heritable resistance of different bacteria to multiple antibiotics. Moreover, it also increases campylobacter resistance to antimicrobials. Alpha-tocopherol represents a new alternative approach against bacterial resistance. It shows modulatory activity on efflux system, showing clinically relevant results. Therefore, it is important to study different resistance mechanisms of campylobacters.
Campylobacter spp,Antibiotic resistance,Efflux Pump Inhibitors,α-tocopherol
https://zvjz.journals.ekb.eg/article_28652.html
https://zvjz.journals.ekb.eg/article_28652_1282504a228fbf6c13d3785c8011e3d1.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Economic Assessment of Various Levels of Protein and Energy in Pigeon Squabs Diet
20
30
EN
Mohamed A.E.
Omar
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egy
omarkafafy@yahoo.com
Fardos A.M.
Hassan
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Sara E.
Shahin
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2019.28653
This study was conducted to evaluate the effect of nine diets through the application of 3×3 randomized design which included three levels of energy (2600, 2800 and 3000 Kcal/kg) with 3 supplemental protein levels (14, 16 and 18%) on growth performance, blood parameters, carcass yields and economic efficiency of Baladi pigeon squabs from 28 days old until 6 months of age (age of sexual maturity). A total of 135 pairs of squabs were randomly and independently allocated to the nine dietary treatments, each containing 15 pairs (3 replicate of 5 pairs). The diets were formulated in mash form fortified with vitamin and mineral premix. Moreover, feed and water were provided for <em>ad-libitum</em> consumption. The results showed that, squabs fed higher energy and crude protein diets (3000 ME+16% CP) had higher live body weight with a coincident significant decrease of the feed intake (P<0.0001). In addition, dressing (%), breast and thigh meat yield (%) increased significantly with increasing levels of both energy and protein, where they hit the peak values in squabs fed on 3000 ME+16% CP diet. Furthermore, increasing energy levels did not affect serum proteins (albumin and globulin), urea and uric acid. However, it had a significant positive effect on serum lipids (cholesterol, triglyceride, high density lipoprotein and low density lipoprotein). In terms of economic analysis, 3000 ME+16% CP diet revealed the lowest cost per Kg live weight of birds 81.87 L.E per pair, along with the highest economic return 110.3 L.E per pair and net profit 10.43 L.E per pair. In conclusion, squabs can efficiently utilize diet containing high energy (3000 Kcal/kg) and crude protein (16%) diets, consequently, it can be used to optimize their growth performance and maintain the maximum economic return
Pigeons growth performance,crude protein,Metabolic Energy,Economics of squabs
https://zvjz.journals.ekb.eg/article_28653.html
https://zvjz.journals.ekb.eg/article_28653_03fecf7de5f6f09b02515bab408424e8.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
The Potential Effect of Circadian Rhythms and Lighting Duration on Behavior and Growth Performance of Quails under Egyptian Condition
31
36
EN
Hesham
H
Mohammed
Animal Behaviour & Hygiene Department, Faculty of Vet Med, Zagazig University
heshamvet_hosny@yahoo.com
10.21608/zvjz.2017.29238
The aim of this study was to observe and record the changes in behaviors and performance of Japanese quails "Coturnix japonica" due to circadian rhythms and different light duration. A total of 220 Japanese quail chicks were used and distributed to two experiments (the circadian rhythms (100 birds) and the lighting duration study (120 birds). The behaviors were recorded by direct observation and video recording. The results showed significant differences in the most recorded behaviors among the different day times, especially in video recording. The ingestive and kinetic behaviors were significantly higher at morning, while the resting behavior was the highest in mid-day. Comfort behavior was significantly lower at morning than other times. The light duration had significant effects on ingestive and kinetic behaviors. It was highest in 24 hours of light duration. While, the dust-bathing was significantly higher in 14 (2.9) than 24 hours of light duration (1.74). Quails growth performance did not reveal significant differences between quails kept in 14 and 24 hours of light duration. It was concluded that, the quails on different day times and lighting duration showed significant difference on the normal behavior
Quail,Behavior,Lighting Duration,Circadian Rhythms
https://zvjz.journals.ekb.eg/article_29238.html
https://zvjz.journals.ekb.eg/article_29238_fd255648b87dcbc120c4c503dcd7dfca.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Utilization of RT-PCR and Restriction Enzyme Analysis in Detection and Differentiation of Pigeon Paramyxovirus-1 and Newcastle Disease Virus in Pigeons
37
47
EN
Esraa E.
Hamouda
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
Nagi A.A.
Mohamed
Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
Amal
A M
Eid
0000-0003-4183-4899
Avian and Rabbit Diseases, Faculty of Veterinary Medicine, Zagazig University, Sharkia, Egypt
amalaeidvet@gmail.com
Abdelshakour N.A.
Ismail
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
Mohamed A.
El-Sisi
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
Ola A.A.
Hassanin
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
Naser A.
Abou-Hashem
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 45511, Egypt
10.21608/zvjz.2017.29239
Fifty-one pigeon houses from both commercial lofts and backyard of different ages and breeds were investigated for incrimination of pigeon paramyxovirus-1 (PPMV-1) and/ or Newcastle Disease virus (NDV) during 2013-2015. The results of clinical examination revealed nervous signs (wing paralysis, head tremors, torticollis, opisthotonos and leg paralysis), greenish diarrhea, and respiratory signs, with variable mortalities. The gross lesions included congestion and hemorrhage in brain; nephrosis and/ or nephritis in kidneys, ulcer in intestine and mucoid enteritis. Virus isolation via chicken embryo inoculation revealed embryo congestion and hemagglutination (HA) activity of harvested allantoic fluids were detected 41 samples (80.39%) with HA titers ranging from 2<sup>6</sup>- 2<sup>10</sup>. The hemagglutination inhibition (HI) results of the same isolates against PPMV-1 and NDV hyper immune sera which prepared in rabbits (1:5dilution) varied from 2<sup>3</sup> – 2<sup>10 </sup> in 38 virus isolates. The allantoic fluids of 41 haemaglutinating virus isolates were subjected to Reverse transcriptase polymeraze chain reaction (RT-PCR), Thirty-eight isolates evidenced successful products for partial amplification of fusion protein gene of NDV and PPMV-1 at 356 bp using specific primers. Restriction fragment length polymorphism (RFLP) using a set of three enzymes; HhaI, MspI and MboI. revealed that 9 strains (mesogenic / lentogenic PPMV-1), 27(mesogenic / lentogenic NDV), one velogenic strain for PPMV-1 and the other was NDV. MDT value in ECE revealed 72 ± 0.0 and 62.4±5.89 indicating that both isolates could be mesogenic. It could be concluded that both NDV and PPMV-1 are co- circulating among pigeons and causing economical losses. Rt-PCR followed by RFLP can be useful rapid tools for detection and identification of both viruses. Beside, in the view of the failure of adopted prophylactic vaccination, both LaSota and Inactivated PPMV-1 vaccines must be included to overcome the impact of aforementioned disease problems in pigeons
PPMV-1,NDV,Pigeons,PCR,differentiation,RFLP
https://zvjz.journals.ekb.eg/article_29239.html
https://zvjz.journals.ekb.eg/article_29239_f9b3b2b5e17e789b072615ca459d180e.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Bacteriological and Molecular Characterization of Salmonella Species Isolated from Humans and Chickens in Sharkia Governorate, Egypt
48
61
EN
Heba
Ahmed
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University
heba_ahmed@zu.edu.eg
Rasha M.
Gharieb
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohamed E.M.
Mohamed
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Magda A.
Amin
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Rehab E.
Mohamed
Zoonoses Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.29240
This study was carried out to investigate the occurrence of <em>Salmonella </em>species in 690 different samples collected from Sharkia Governorate, Egypt. The samples comprised of chicken meat, organs, eggs, cloacal swabs and wooden surface swabs from pluck shop outlets and hand swabs from workers. In addition, stool swabs from patients with gastroenteritis attending the Outpatient Clinics at Zagazig and Abo-Hammad cities. <em>Salmonella </em>spp. were detected in 5.9%, 2.6%, 4.2%, 10.4%, 10% and 10% of chicken meat, organs, egg shell, cloacal swabs, surface swabs and hand swabs, respectively. The isolation rate from stool swabs was 0.8%. Biochemical Identification revealed that <em>Salmonella </em>spp. were identified in 129 out of 690 examined samples (18.7%). Meanwhile, molecular identification using <em>inv</em>A gene revealed that only 29 <em>Salmonella</em> isolates were detected (4.2%).<em> S.</em> Typhimurium and <em>S. </em>Enteritidis were identified from different samples with the percentages of 1.2 and 1.01, respectively. The other identified <em>Salmonella </em>serovars were <em>S.</em> Newport (0.9%), <em>S.</em> Kentucky (0.7%), and <em>S. </em>Infantis (0.4%). Virulence associated genes including <em>avr</em>A, <em>hil</em>A and <em>pef </em>were identified in 100, 91.3 and 10.3% of the examined isolates. In conclusion, a proportion of chicken carcasses and giblets sold in Sharkia Governorate, Egypt was contaminated with <em>Salmonella</em> spp. including potentially virulent <em>S</em>. Typhimurium and <em>S</em>. Enteritidis, posing risk for human consumers.
Salmonella,Serotyping,PCR,avrA,hilA,pef
https://zvjz.journals.ekb.eg/article_29240.html
https://zvjz.journals.ekb.eg/article_29240_5384ef8922d8ffa17d366b3d5bf317c4.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Evaluation of Carbon Fiber and Bovine Pericardium Implantation in Tenorrhaphy of Common Calcanean Tendon Rupture in Goats
62
71
EN
Ali E.
Kandeel
Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt/Biology Department, Faculty of Sciences and Arts, University of Jeddah, Alkamil Branch, KSA
Mahmoud A.
Abdraboh
Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abd El- Basit M.
Abd El Aal
Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Shimaa A.
Ezzeldein
0000-0002-6072-7207
Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
shimaa.ezzeldein@yahoo.com
10.21608/zvjz.2017.29241
Common calcanean tenorrhaphy was carried out on right hind limbs of 18 goats after removal of two cm segment from the tendon using linen (nylon) No. one and locking loop suture pattern alone (group I) or together with carbon fibers implantation (group II) or with bovine pericardial flap (group III). Surgery was done under epidural analgesia and the tibiotarsal joint of the operated limb was immobilized using cortical screw 3.5mm with application of full limb plaster cast<em>. </em>Post-operative healing of the tendon was monitored through clinical observation, ultra-sonographic, macroscopic and microscopic examinations at intervals (3, 9, 12 weeks) post operatively. With the use of bovine pericardial flap, the operated animals showed earlier improvement compared with the others. Bovine pericardial flap also induced early tendon healing and better organization of tendon fibers.
Common Calcanean Tendon Rupture,Tenorrhaphy,goats,Bovine Pericardium
https://zvjz.journals.ekb.eg/article_29241.html
https://zvjz.journals.ekb.eg/article_29241_acc177df7c986b5243ac75c24eee59ac.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Effects of feeding Aloe Vera Leaves Powder on Performance, Carcass and Immune Traits of Broiler Chickens
72
78
EN
Seham M.
Mohamed
Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
sehamyousef2011@yahoo.com
Wafaa. A.
El-Eraky
Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mahmoud. F.
Al- Gamal
Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.29242
The current study was carried out to study the effect of dietary supplementation with Aloe Vera leaves powder (AVLP) as natural feed additives on growth performance, carcass characteristics and immune response of broiler chickens. Ross unsexed one day old (75 gm) broiler chicks (n=90) were randomly divided into five experimental groups; each group with three replicates (18 chicks/group; 6 chicks/replicate). Diets supplemented with AVLP at rates of 0, 1.5, 2.0 and 2.5 % and the last group supplemented with antibiotic (Oxytetracyclin 1 g/kg diet) for 42 days. Body weights and feed residues were monitored weekly. At the end of the trial, three birds/group were euthanized and then blood samples were collected, moreover carcass dressing and visceral organs were collected and weighed. Results revealed that AVLP had no significant (P>0.05) effects on growth performance of broiler chicken. All treated groups showed non-significant (P>0.05) effect on internal organ weights and carcass traits. Diets supplemented with 1.5, 2.0 and 2.5 % of AVLP stimulate the immunity by increasing the phagocytic capacity and antibody titre against Newcastle disease virus when compared with the control group. These results indicated that addition of AVLP in broiler chicken’s diets has no adverse effects on growth performance beside it improves the humoral and the cellular immunity
Aloe Vera Leaves Powder,broiler,performance,carcass,immunity
https://zvjz.journals.ekb.eg/article_29242.html
https://zvjz.journals.ekb.eg/article_29242_89015432973a80bbff07e488b0b4f554.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Occurrence of Avian Influenza Virus (H9 subtype) in Broiler and Layer Chickens at Sharkia Governorate
79
90
EN
Hossam A.A.
Gado
Reference Laboratory for Quality Control on Poultry Production (RLQP). Animal Health Research Institute, Sharkia Branch, Egypt
Amal A.M.
Eid
0000-0003-4183-4899
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
amalaeidvet@gmail.com
Ibrahim A.
Ghanem
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohamed K.
Moursi
Reference Laboratory for Quality Control on Poultry Production (RLQP), Animal Health Research Institute, Ismailia Branch, Egypt
10.21608/zvjz.2017.29243
Avian influenza (AI) is a contagious disease caused by type A influenza viruses. In spite of the mild nature of H9N2 low pathogenic AIV, the virus was isolated from frequent disease outbreaks with high mortality in different parts of the world. In this study, forty-two layer and broiler chicken farms (21/each) suspected to be infected with AIV of different breeds and ages during 2012 - 2014 were examined at Sharkia Governorate. The occurrence of AI in chickens using HI assay was 40.47%. Subtyping for H9 and H5 subtypes has been done by specific antisera. The H9 was detected in 30.95% and H5 was 9.52%. However, the occurrence of NDV in chickens was 23.80%. The selected seven AIV isolates representing broiler and layer flocks were confirmed by rRT-PCR using primers and probes specific for Matrix gene, H9 gene, H5 gene and H7 gene. Seven isolates were positive for M. gene indicating AI virus detection, and then 6 out of 7 isolates were positive for H9 gene but negative for H5 and H7 genes. The last isolate was positive for H5 gene, negative for H9 and H7 genes. The phylogenetic analysis for HA gene of the Six H9 AIV isolates showed the placement of the Egyptian viruses within the same lineage of H9N2 viruses that circulated in the region with the parent strain (A/Quail/ Egypt/ 113413v/2011) of G1/97 lineage and other strains that circulating in the Middle East.The circulating H9N2 AIV genetic analysis is pointing to a minor evolution of H9N2 viruses. But at the same time the co-circulation of H5 & H9 subtypes of AIV could be a risk for emergence of new AI viruses. Also, concurrent infection with viral (IBV and NDV) as well as bacterial agents (MG, Salmonella and <em>E. coli</em>) are considered the main cause behind increased mortality in H9N2 positive flocks.
Avian influenza virus,Low pathogenic-H9-Sequencing
https://zvjz.journals.ekb.eg/article_29243.html
https://zvjz.journals.ekb.eg/article_29243_80164c51ddce7eac74f0b8946bfa1b14.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Prevalence of some Enteric Bacterial Infections Causing Rabbit Enteritis and Attempts to Control Rabbit Coli Enteritis with Phytobiotics
91
101
EN
Ahmed, K.
Ismail
Diagnostic Veterinary Hospital, Hussania, Sharkia
Hanan, M.F.
Abdien
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University
hanan_24395@yahoo.com
Dalia, M.
Hamed
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University
Wail M.K.
El Feil
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Suez Canal University
10.21608/zvjz.2017.29244
A field survey was conducted to monitor the prevalence of some enteric bacterial infections incriminated in rabbit enteritis outbreaks. Ninety bacterial isolates were recovered from diseased /or freshly dead rabbits with a history of severe diarrhea representing four farms in El-Sharkia Province. The predominant isolates were <em>E. coli </em>(24.29%),<em> Klebsiella </em>(14%), <em>Proteus miriables</em> (2.33%) and <em>Proteus vulgaris</em> (1.4%). All bacterial isolates were highly sensitive to levofloxacin. Isolated <em>E. coli </em>was later identified by using PCR. Fifty-three recently weaned White New Zealand rabbits were experimentally used to monitor the efficacy of two herbal extracts and probiotics (Healthy gut®; Immuplant plus®) supplementation with/or without enrofloxacin treatment in controlling artificial infection of the pathogenic streptomycin adapted <em>E. coli</em> isolate strain in a rate of (2.5 x 10<sup>10 </sup>CFU/0.1 mL/ rabbit orally) and improvement of growth parameters. Our results showed that; treatment with two (Healthy gut®; Immuplant plus®) induced an improvement in general health conditions and growth performance parameters with significant decrease in total labeled pathogenic <em>E. coli</em> shedding of infected supplemented group. In addition, this supplementation when followed by treatment with enrofloxacin after <em>E. coli</em> infection refluxed significant reduction of bacterial shedding with improvement of mean body weight and feed conversion ratio when compared with all other treated groups
Rabbit,E. coli,diarrhea,Herbal
https://zvjz.journals.ekb.eg/article_29244.html
https://zvjz.journals.ekb.eg/article_29244_270a3d0bc49e96930d49c8f02c36e326.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Evaluation of Egg Quality of Japanese Quail in Different Housing Systems
102
108
EN
Hesham
Elsayed
Veterinary Public Health Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
heshamvet_hosny@yahoo.com
Heba S.A.
Gharib
Veterinary Public Health Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2019.28654
A total of 135 birds of Japanese quails at 5<sup>th</sup> week of age were divided randomly into three groups housed in galvanized metal cages, pens covered with fine and coarse sawdust for the 1<sup>st</sup>, 2<sup>nd</sup> and 3<sup>rd</sup> groups, respectively. The results revealed that egg length, egg shape index, shell weight, shell ratio and egg thickness were significantly affected by housing system. The egg produced in fine sawdust group had the highest egg shape index (82.75 %), shell weight (1.69 g), shell ratio (15.88 %) and shell thickness (0.27 mm) with lowest egg length (29.98 mm) in fine sawdust group when compared with other systems. On the other hand, egg weight (11.99 g), egg surface area (22.91 cm<sup>2</sup>), egg width (25.86 mm) was higher in the cage system with no significant differences. Moreover, the most of internal egg quality was significantly affected by different housing systems, while there were no significant differences in albumin ratio and width, yolk weight and ratio. Threfore, the egg quality (internal and external traits) was better in cage system and deep litter system with fine sawdust in comparison to the deep litter system with coarse sawdust.
housing system,Quail,egg quality
https://zvjz.journals.ekb.eg/article_28654.html
https://zvjz.journals.ekb.eg/article_28654_40f542a165e805777c5d1495df7c1fb9.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Prevalence of Gastrointestinal Nematodes and the Role of Allicin in Treatment of Cattle in Sharkia Governorate
109
117
EN
Elshaima
Mohamed VVVV
Fawzi
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
elshaimafawzi@yahoo.es
Ibrahim
Elsohaby
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2019.28655
The objectives of this study were to investigate the prevalence of gastrointestinal nematodes (GIN) among cattle in Sharkia Governate, Egypt and to determine the efficacy of allicin as a treatment for GIN infection. Fecal samples (n = 113) were collected randomly from cattle at different localities in Sharkia Governate between September 2016 and August 2018. Samples were then examined parasitologically. Overall, GIN were found in 21 (18.6%) of the samples, the highest GIN prevalence was observed in winter (25.6%), while the lowest prevalence was in summer (10.3%). Adult cattle (42.9%) showed higher GIN infection than young calves (23.8%). Further, most of the infected cattle (92.4%) showed a light intensity of infection with GIN. The coproculture of infected fecal samples reveled that, 15 (71.4%) samples have single infection and 6 (28.6%) samples have mixed infection and <em>Trichostrongylus </em>spp. (41.9%) were the most frequently observed GIN, followed by <em>Strongyloides </em>spp. (35.2%), whereas, <em>Cooperia </em>spp. (8.7%) was the less frequently observed GIN. No significant difference (<em>P</em> = 0.78) was observed between using albendazole and allicin for treatment of GIN in cattle. The current study provides new and updated results that may help in the effort to control GIN. Also, this study shows that allicin could be used for treatment and control of GIN in cattle. However, further investigations in the efficacy of allicin in treatment of GIN in cattle and actual dose, are warranted.
Gastrointestinal Nematodes,Allicin,cattle,Egypt
https://zvjz.journals.ekb.eg/article_28655.html
https://zvjz.journals.ekb.eg/article_28655_e2e5c4379c74aad78dc21cc1e5fe2b2c.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Evaluation of Dietary Inclusion of Mango Kernel Meal and Oat Extract on Performance and Immunity of Oreochromis niloticus
118
125
EN
Walaa
El-Houseiny
Fish Diseases and Management Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abdelhakeem
El-Murr
Fish Diseases and Management Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
hakimelmor@hotmail.com
Badawi, M.
El-Sayed
Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2019.28656
The aim of the current study was to evaluate the effect of dietary supplementation with mango(<em>Mangifera indica</em>) kernel meal (MKM) andoat(<em>Avena sativa</em>) extract (OE) on growth performance, some serum biochemical parameters, immune status and protection of <em>Oreochromis niloticus</em> against <em>Aeromonas hydrophila</em> infections. A total of 180 <em>Oreochromis niloticus</em> fingerlings were divided to three experimental groups (each of 60 and each group was divided into four aquaria, 15 fish/aquarium); The first group was fed the control diet (without any additive) and the second group was fed diet supplemented with MKM, while the third group was fed diet supplemented with OE for 60 days. Growth performance and immunological parameters (lysozyme, immunoglobulin M) were examined at 2 months of age. Fish were challenged by<em> A. hydrophila</em> and the mortality were recorded after 14 days post-challenge. Fish fed diet contained OE had a higher final body weight, body weight gain and body weight gain percentage when compared with other groups. Immunological parameters estimated in this work were significantly improved (P<0.05) in MKM and OE fed groups when compared with the control group. Survivability after 14 days post-challenge was lower in control group (60%) and higher in other dietary groups. In conclusion, MKM and OE improved the growth performance, blood constituents, immune status and enhanced the challenging ability of <em>Oreochromis niloticus</em> against <em>A. hydrophila</em>.
Nile tilapia,Mango Kernel Meal,Avena Sativa Extract,performance,immunity
https://zvjz.journals.ekb.eg/article_28656.html
https://zvjz.journals.ekb.eg/article_28656_8efa31fea112f49436b0e3cb61d081b0.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Ameliorative Effects of Curcumin-Zinc Oxide Nanoparticles Conjugate on Cyclophosphamide-Induced Infertility in Male Rats
126
132
EN
Khalifa E.
Ahmad
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University,
44511, Egypt
Reda M.
Abd El-Aziz
Physiology Department, Faculty of Veterinary Medicine, Zagazig University,
44511, Egypt
redaabdelazziz81@yahoo.com
Mahran M.
Abd El-Emam
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University,
44511, Egypt
10.21608/zvjz.2019.28657
on testicular tissue damage induced by the anticancer cyclophosphamide. Seventy adult male albino rats weighing 150±15g were used in this study; 10 of them served as a control and 60 rats were intraperitoneal injected with cyclophosphamide at a dose of 30 mg/kg BW every other alternative day for 14 days. They were subdivided into CP group, CP + Curcumin NPs (15 mg/kg B.W), CP + ZnO NPs (10 mg/kg B.W), CP + Curcumin-ZnO NPs conjugate (10 mg/kg B.W), CP + Curcumin-ZnO NPs conjugate (15 mg/kg BW) and CP + Curcumin-ZnO NPs conjugate (20 mg/kg B.W) group. All treatments were given for 30 days. The results reported that curcumin- ZnO NPs conjugate treatment at doses of 15 and 20 mg/kg BW exhibited significant increase in sperm motility percent, sperm count, testicular antioxidant enzymes activities and serum testosterone and FSH levels and significant decrease in sperm abnormalities percent compared to cyclophosphamide treated rats. It was concluded that curcumin- ZnO NPs conjugate at doses of 15 and 20 mg/kg B.W ameliorate the testicular tissue damage induced by the anticancer cyclophosphamide
cyclophosphamide,Curcumin-ZnO,NPs Conjugate,Oxidative Stress,Rat testes
https://zvjz.journals.ekb.eg/article_28657.html
https://zvjz.journals.ekb.eg/article_28657_6b0ac3b6ad93231fbcc1362d61fe7a29.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Some Studies on Swollen Head Syndrome in Broiler Chickens in Egypt
133
141
EN
Mohamed M.
Shawki
Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
drshawkyvet2006@yahoo.com
Mohamed A.
Lebdah
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abeer M.
Shahin
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Samir A.
Nassif
Central Laboratory for Evaluation of Veterinary Biologics (CLVEB), Abbasia, Cairo
10.21608/zvjz.2019.28658
Swollen Head Syndrome (SHS) is a disease of upper respiratory tract affecting broilers and broiler breeders which resulted in inflammatory exudates beneath the skin<strong>.</strong> SHS has been described as a multi-factorial disease where the initial lesion mainly caused by avian Metapneumo virus (aMPV), while the clinical signs are a consequence of bacterial complications and the severity of the disease depends on environmental factors<strong>.</strong> This study was planned to detect and try to isolate the etiological agents of SHS from 40 broiler flocks in 3 Egyptian Governorates (Sharkia, Dakahlia and Damietta). The chickens incorporated swollen heads and respiratory manifestations as nasal discharges, coughing, tracheal rales and frothy conjunctivitis from different localities. Samples included trachea and lung tissues, choanal cleft swabs and scraps from sinuses and turbinates. The AMPV subtype B was detected in 5 flocks by Real Time Polymerase Chain Reaction (RRT-PCR) -using 2 specific probes for differentiation of subtypes A and B. the AMPV-subtype B was detected with the percentage of 12.5%. Bacterial examination of collected samples revealed presence of mixed infection in some of examined flocks with isolation of <em>E. coli </em>in a percentage of 70%; <em>Proteus mirabilis</em> 40% and <em>Pseudomonas aeruginosa</em> (25%). In conclusion, to our knowledge, this is the first detection of aMPV among broiler chickens suffering from SHS in Egypt, using RRT-PCR in concurrent bacterial infections with <em>E. coli, P. mirabilis </em>and<em> P. aeruginosa</em>.
Swollen Head Syndrome,Broilers,Avian Metapneumo Virus
https://zvjz.journals.ekb.eg/article_28658.html
https://zvjz.journals.ekb.eg/article_28658_89bbea6568ec67861b6546cac64d68ce.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Genetic Parameters, Breeding Values and Genetic Trends for Some Productive and Reproductive Traits of Holstein Cows in Egypt
142
154
EN
Tamer M.
Abdel-Hamid
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
drtamoabha18111980@gmail.com
Khairy M.
El-Bayoumi
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mahmoud S.
El-Tarabany
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Wafaa R.I.A.
Sherief
Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2019.28659
This study aimed to estimate the genetic parameters (heritability and genetic correlation), breeding value and genetic trends for some productive and reproductive traits of Holstein Friesian cows in Egypt with animal model statistical method. A total of 3400 lactation records collected from 1060 cow's progeny of 99 sires and 691 dams through the period from 1998 to 2010 were used in this study. Results of genetic correlations showed great variability from very high positive to very low negative values. High heritability estimates were recorded for all studied traits with the exception of days in milk, calving interval and days open which revealed low estimates. The ranges of cows breeding values have been found to be higher than either sires or dams for all studied traits. Genetic trends were positive for 305-day milk yield (2.68 kg), age at first calving (0.020), fat yield (0.031kg), fat% (0.001%) and protein % (0.002%), but were negative for protein yield (-0.098 kg) and days open (-0.219). In conclusion, higher range of the cow breeding values than either sires or dams revealed a wider genetic variation and a good opportunity for selecting the superior cows. Positive genetic trends for 305-day milk yield, age at first calving, fat%, fat yield and protein% indicated that there was a genetic improvement in these traits in desirable direction, but negative genetic trends for days open and protein yield indicating a decrease in mean breeding value for these traits over the study period.
Holstein,calving interval,Breeding value,Genetic trend,animal model
https://zvjz.journals.ekb.eg/article_28659.html
https://zvjz.journals.ekb.eg/article_28659_3c846178f83b4a59f78cfc6013188672.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Experimental Pathological Studies on Ochratoxicosis in Broiler Chickens
155
164
EN
Abd El-Moneim A.
Ali
Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Nahla A.
Refat
Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Rehab E.
Mowafy
Pathology Department, Animal Health Research Institute, Zagazig Branch
mowafyrehab@yahoo.com
Safaa A.
Gaheen
Pathology Department, Animal Health Research Institute, Zagazig Branch
10.21608/zvjz.2019.28660
Forty broiler chicks (Hubbard breed), two weeks old were used to study the pathological lesions and residues of ochratoxin A in some chicken organs. Experimental chicks were divided into two groups. First group (30 chicks) were fed on ration contaminated with 100ppb /kg ration, while second group (10 chicks) were kept as control and fed on ration free from OTA till the end of the experiment. Fifteen chicks from group 1and five chicks from group 2 were sacrificed 28 and 36 days post feeding (PF). The clinical signs, mortalities and lesions in addition to OTA residues were detected and recorded. The chicks in group 1 showed 20% mortalities beside diarrhea, dehydration and emaciation. The kidney lobules appeared pale with urates deposits due to intense nephrotic changes, nephritis, fibrosis and gout. The liver showed hepatic hemorrhages apoptosis, necrosis and hyperplastic bile ductules. Intense lesions in brain in the form of edema, meningeal lymphocytic cells aggregations, degenerated neurons and purkinje cells were recorded. GIT lesions (proventriculus and intestine) due to direct contact with OTA contaminated ration were also seen. OTA residue was higher in liver than kidneys while the lowest value was detected in skeletal muscle. It could be concluded that OTA induced neurotoxicity beside hepato-renal toxicity and GIT lesions due to its toxic effect and its adverse effect on growth performance.
ochratoxins,residues,Carcinogen,Teratogen
https://zvjz.journals.ekb.eg/article_28660.html
https://zvjz.journals.ekb.eg/article_28660_4c9fccde50b9ba523876d2a12ac73b79.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Studies of Reproductive Changes during Pregnancy in the Bitch
165
171
EN
Faten M.
Labib
Theriogenology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
El Azab A.
El Azab
Theriogenology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Hassan H.
Mansour
Theriogenology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Atef B.
Mahdy
Theriogenology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Asmaa A.
Abdallah
Theriogenology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
10.21608/zvjz.2019.28661
In the present study, seven mongrel bitches were used, their age ranged from 15 months to 3 years. Vaginal cytology was carried out during pregnancy period of these bitches by taking vaginal smears weekly to investigate vaginal cytological changes. Serial ultrasonography was performed daily on these bitches from the 15<sup>th</sup> day after the last breeding until birth day to determine the time of appearance of different gestational structures. The first observation of the extra-fetal and fetal structures was as follows: The yolk sac membrane was first detected as an echogenic U-shaped fetal membrane in a range of 24-25 days. A third echogenic fetal membrane, allantoic membrane, was first detected on Day 27. On Day 23, in the uterine wall surrounding the gestational sac, an apparently hyperechoic inner layer was differentiated to the zonary placenta which appeared as two thick bands one on either side of the fetus on Day 29. The embryo appearance was on Day 20.5. The early time of the position of the embryo either opposed to the uterine wall or dependent on chorionic cavity was nearly on Day 22 and on Day 24, respectively. The heartbeat, which is one of fetal vital signs, was detected as a bright echogenic flickering motion on Day 21. On Day 26 in mongrel bitches, the features of the embryo changed from an oblong to bipolar shape and form two loops of number 8, size and density the same. An anechoic area in the head of the embryo was detected on Day 29. The limb buds were firstly detected on Day 29. Fetal movement was firstly detected on Day 33. Meanwhile, the skeleton of the fetus was first detected as hyperechoic structure on Day 33.
Reproduction,Pregnancy,Bitch,Ultrasonography
https://zvjz.journals.ekb.eg/article_28661.html
https://zvjz.journals.ekb.eg/article_28661_0ce44a32168dfa681fdd7ae5f48a98c8.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Some Pharmacological Studies of Ciprofloxacin and Levofloxacin in Rats
172
180
EN
Abdel-Alim F.
Abdel-Alim
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohammed A.
Kamel
0000-0003-3430-1648
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
kamelma78@gmail.com
Refaat A.A.
Elsayed
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.28662
This study was conducted to investigate some pharmacological effects of ciprofloxacin and levofloxacin in their therapeutic and double therapeutic doses on healthy male albino rats. Forty five rats were divided into 5 groups, each of 9 rats, 1<sup>st</sup> was left as a control, 2<sup>nd</sup> and 3<sup>rd</sup> groups received 100 mg/kg BW ciprofloxacin and levofloxacin respectively, orally for 10 successive days (therapeutic dose). The 4<sup>th</sup> and 5<sup>th</sup> groups were administrated 200 mg/kg ciprofloxacin and levofloxacin respectively, orally for 10 successive days (double therapeutic doses). Urine analysis and serum biochemical parameters were estimated in addition to histopathological examination of liver and kidneys. The urine analysis revealed that levofloxacin at its therapeutic and double therapeutic doses produced a significant increase in urinary proteins, blood, bilirubin, urobilinogen and urinary ketones while ciprofloxacin doses produced less damage. However, serum biochemical parameters disclosed that levofloxacin is a safer fluoroquinolone than ciprofloxacin; these results were confirmed by histopathological examination of liver and kidneys. Urine analysis despite widely used in human laboratories, it is not a reliable technique; blood biochemical parameters examination and histopathological examination are more dependable.
ciprofloxacin,Levofloxacin,urinalysis,Liver and kidney functions
https://zvjz.journals.ekb.eg/article_28662.html
https://zvjz.journals.ekb.eg/article_28662_81f5b31f367c0f6ac83f3797f3cf8ea6.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Assessment of the Duration of Maternal Antibodies Specific to Live Attenuated peste des petits Ruminant Virus Vaccine in Lambs and Kids
181
188
EN
Ayman A-
Shehata
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Elshaima M.
Fawzi
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
elshaimafawzi@yahoo.es
Afaf M.
Menaze
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohamed I.
Essa
Animal Medicine Department, Division of Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mervat M.
Mahmoud
Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt
10.21608/zvjz.2017.28663
Maternal immunity in lambs and kids born from vaccinated dams with <em>peste des petits </em>ruminant (PPR) live attenuated cell culture vaccine was evaluated by using competitive ELISA (c-ELISA). Serum samples were collected from each dam prior to the vaccination to detect the titre of antibody against the infection with PPRV and from lambs and kids on the day of birth, then every 15 days until reach 4.5 months of age to determine the level of maternal antibodies against PPR virus transferred from vaccinated dams to their newly born lambs and kids. Results showed that the antibody titer averages in lambs and kids reach the maximum at one month of age (OD average below 20) then, declined gradually at 105 days in which 80 % of kids still have OD average below 50while 40% of lambs are still protected. So, we recommended the application of vaccine to newly born lambs and kids from immunized dams at 3 months of age by live attenuated PPR vaccine strain Egypt/87 grown on the Vero cell culture.
Small ruminants,PPRV- vaccine,maternal immunity,c-ELISA
https://zvjz.journals.ekb.eg/article_28663.html
https://zvjz.journals.ekb.eg/article_28663_de6b07c9060ec46ba567b6207c5ba204.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Oxidative Stress Biomarkers in Dystrophic Lambs and Kids in Relation to Body Condition Scores
189
200
EN
Yasmin H.
Bayomi
Animal Medicine Department (Internal Medicine), Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
yasmin.dream@yahoo.com
Wafaa A.M.
Mohamed
Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abdel Karem M.
Morsi
Animal Medicine Department (Internal Medicine), Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.28664
This study was conducted to investigate the relationship between the severity of muscular dystrophy, caused by vitamin E and selenium deficiencies in lambs and kids, and their body condition score (BCS). A total of 71 lambs and kids aged from 1-6 months were used; 51 dystrophic animals (38 lambs and 13 kids) and 20 healthy ones (10 lambs and 10 kids). The animal grouping was done on the basis of BCS and the obtained hematological and biochemical alterations. Blood samples were collected after clinical examination to all animals, for performing the clinicopathological studies. Results showed that the incidence and severity of the disease were higher in rapidly growing and well-nourished lamb (BCS 3.5-4), while the occurrence of the disease in kids was more common in BCS 1-2.5. Regarding biochemical findings, the serum vitamin E, Se, CAT and GSH-Px recorded lower values in dystrophic lambs and kids than the control, while higher values of CPK, CTn I, MDA and NO were recorded with the strongest alterations in good bodily conditioned ones. A significant decrease in serum Mg level in dystrophic kids with BCS 1-3 was also observed. In conclusion, proper nutrition of dam and young without shortage or excess is necessary. Also, vitamin E and se supplementation should be increased in good bodily conditioned and rapidly growing animals than their counterparts to activate the antioxidant system and reduce oxidative stress.
BCS,lambs,kids,Vitamin E deficiency,Oxidative Stress
https://zvjz.journals.ekb.eg/article_28664.html
https://zvjz.journals.ekb.eg/article_28664_6c4643b68c146ecb2a51cdadccbc7f04.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Effect of Zinc, Selenium and their Combination on Cadmium- Induced Oxidative Stress in Rat Kidney-A Molecular Study
201
209
EN
Safaa I.
Khater
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Hamad A.
ELsaadawy
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Shrouk A.
El-Basyoni
Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
dr_chrry54@yahoo.com
10.21608/zvjz.2017.28665
Cadmium (Cd) is considered as a very toxic heavy metal which transfers to the body through many routes as food, water and air. Zinc (Zn) and selenium (Se) have a great role in alleviation of Cd toxicity in kidneys. This study aimed to look into the interaction between Cd, Zn, Se and their effects on gene expression of the antioxidant enzymes in the kidneys of rats. Fifty male adult albino rats were divided into five groups (n=10) that received orally the following doses daily for one month: Group I: control group received normal saline, Group II: received 2mg/kg BW CdCl<sub>2</sub>, Group ΠI: was administered 2mg/kg CdCl<sub>2</sub> and 2mg/kg ZnCl<sub>2</sub>, Group IV: received 2 mg/kg CdCl<sub>2</sub> and 0.23 mg/kg Na<sub>2</sub>Seo<sub>3</sub>, and Group V: received 2 mg/kg CdCl<sub>2</sub>, 2 mg/kg ZnCl<sub>2</sub> and 0.23 mg/kg Na<sub>2</sub>Seo<sub>3</sub>). The gene expression levels of SOD1, CAT, GPx, HSP70 and MT1 were assessed in the kidneys; treatment with Cd lowered the expression of antioxidant enzymes (SOD1, CAT, and GPx) and increased the expression level of HSP70 and MT1. Only partial ameliorative effects on the oxidative stress caused by Cd in the kidney have been observed with Se or Zn supplementation during exposure to Cd, while the co-treated with Se and Zn revealed better protection against the observed oxidative stress in kidney.
zinc,selenium,cadmium,Oxidative Stress,Gene expression,Metallothionine
https://zvjz.journals.ekb.eg/article_28665.html
https://zvjz.journals.ekb.eg/article_28665_de094305ddb934d58307a405774b08d7.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Comparative ameliorative effect of Hydrated sodium calcium aluminosilicate and Saccharomyces cerevisiae (Brewer's yeast) against toxic impact of aflatoxin B1 in Oreochromis niloticus (Nile tilapia)
210
220
EN
Abd El-Alim F.
Abd El-Alim
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Azza A. A.
Galal
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
azzapharma@gmail.com
Shahira H.
Mahmoud
Animal Health Research Institute, Zagazig Branch, Zagazig 44516, Sharkia, Egypt
Walaa A.
El-Sayed
Animal Health Research Institute, Zagazig Branch, Zagazig 44516, Sharkia, Egypt
10.21608/zvjz.2017.28666
Different ways have been used in an attempt to decrease the risk of aflatoxicosis in fish. This study was undertaken to compare the possible alleviative effects of hydrated sodium calcium aluminosilicate (HSCAS) and<em> Saccharomyces cerevisiae</em> against the toxic impact of aflatoxin B<sub>1 </sub>(AFB<sub>1</sub>) on<em> Oreochromis niloticu</em>s (<em>O. niloticus</em>). Therefore, 180 normal cultured monosex <em>O. niloticus </em>were randomly allocated into 6 equal groups. Group 1, was received the basal ration only. Group 2, was fed a basal ration supplemented with 0.5% HASCAS. Group 3, was fed a basal diet enriched with 0.25% <em>Saccharomyces cerevisiae</em>. Group 4, was received a diet intoxicated with 2.5 ppm aflatoxin B<sub>1</sub>. Group 5, was fed a diet intoxicated with 2.5 ppm AFB<sub>1</sub> with 0.5% HSCAS. Group 6, was fed a diet intoxicated with 2.5 ppm AFB<sub>1</sub> with 0.25% <em>S. cerevisae</em>. AFB<sub>1 </sub>intoxication induced mortality 16.67 %, leucopenia, lymphopenia, neutrophilia with a significant decrease in phagocytic % and index. Furthermore, significant increases in serum creatinine, ALT and ALP as well as a significant decrease in total protein, albumin and globulin were recorded. Moreover, accumulation of aflatoxin residues in <em>O. niloticus </em>flesh (5 ppb) and liver (15 ppb). While, supplementation of AFB<sub>1</sub> intoxicated diet either with <em>S. cerevisiae</em> or HSCAS ameliorated the drastic effects of aflatoxin on <em>O. niloticus</em> and<em> S. cerevisiae</em> appear to be more effective in the protection of fish from aflatoxicosis than HSCAS.
Aflatoxin B1,residues,hematology,phagocytosis,HSCAS,Saccharomyces cerevisiae
https://zvjz.journals.ekb.eg/article_28666.html
https://zvjz.journals.ekb.eg/article_28666_7c79f80fb633f95fe5b688edb1ef1d53.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Adverse Effects of Memantine
221
234
EN
Gamal El-Din A.M.
Shams
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohamed H.
Khairy
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohamed A.
Saleh
Faculty of Pharmacy, Mansoura University, Mansoura, Egypt
Samar A.
Antar
Pharmacology Department, Faculty of Pharmacy, Horus University
samarantar38@yahoo.com
10.21608/zvjz.2017.28667
The current study covers key issues related to memantine’s toxicity (0.36 mg/kg, P.O. once daily) and its effect on liver, Kidney, blood and antioxidant enzymes for three weeks. Then tissue and blood samples were collected at the 1st, 2nd and 3rd weeks post-treatment. Our results indicated that Memantine has hepato-nephrotoxicity and elevation of liver enzymes (ALT, AST and ALP), creatinine, urea and uric acid. Obvious decline in all blood parameters with respectable decline in antioxidant enzyme like CAT, SOD, GPX and a significant increase in MDA. Histopathology revealed hepatic steatosis and increase the apoptotic and necrotic cells mostly within the centrolobular, as. Also, tubular basophilia and interstitial nephritis were observed. Therefore, Memantine should be used with caution with hepatic or people with kidney problems.
Memantine,Alzheimer’s disease,Dementia,CBC,Antioxidant enzymes
https://zvjz.journals.ekb.eg/article_28667.html
https://zvjz.journals.ekb.eg/article_28667_634ab8eb5f0b8b624906d825385aa1be.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Interaction between Marbofloxacin and Flunixin in Treatment of Pneumonia in Lambs
235
243
EN
Mohammed H.
Khairy
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abd El Alim F.
Abd El Alim
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
fouadabdelalim@yahoo.com
Ibrahim M
Ismail
Animal Health Research Institute, Zagazig branch, Egypt
Mohamed E
Darwish
Animal Health Research Institute, Zagazig branch, Egypt
10.21608/zvjz.2017.28668
Nasopharyngeal swabs were collected from 100 lambs aging 9 -12 months (30 healthy and 70 pneumonic lambs for bacteriological examination. Overall, 25% were positive for <em>P multocida</em>. Antibiogram study of the isolates revealed that marbofloxacin was the highest effective against <em>P. multocida</em>. Forty lambs (20 healthy and 20 naturally infected with <em>P </em><em>multocida</em>) were divided into 8 groups (5, each), the 1<sup>st</sup> group: healthy lambs (control), 2<sup>nd</sup> group: healthy received marbofloxacin, 3<sup>rd</sup> group: healthy received flunixin meglumine, 4<sup>th</sup> group: healthy received marbofloxacin and flunixin meglumine together, 5<sup>th</sup> group: infected non treated, 6<sup>th</sup> group: infected treated with marbofloxacin, 7<sup>th</sup> group: infected treated with flunixin meglumine and 8<sup>th</sup>: group infected treated with marbofloxacin and flunixin meglumine together. Hemato-biochemical changes at 1<sup>st</sup>, 7<sup>th</sup> and 14<sup>th</sup> day post treatment were studied. All clinical sign disappeared and <em>P.multocida</em> microorganisms were not reisolated in group post treated by marbofloxacin alone or together with flunixin meglumine. Healthy lambs received marbofloxacin or flunixin meglumine either alone or together showed significant decrease in RBCs, Hb, PCV%, T protein, albumin, globulin, α, β, γ globulin and significant increase in WBCs, AST, ALT, ALP, GGT, urea and creatinine at1<sup>st</sup> and 7<sup>th</sup> day post injection. Pneumonic lambs showed significant reduction in RBCs, Hb, PCV% T. protein, albumin, total globulin, α, β globulin and significant increase in WBCs, AST, ALT, ALP, GGT, γ globulin, urea and creatinine all over the experimental period. Pneumonic lamb treated with marbofloxacin alone or with flunixin meglumine resulted in complete disappearance of these clinical signs at 1<sup>st</sup> day post treatment but these clinical signs remained in flunixin meglumine alone lambs. It is concluded that, pasteurellosis in lambs resulted in adverse effect in hematobiochemical parameters. Using marbofloxacin alone or together with flunixin meglumine in treatment of diseased lambs lead to improve clinical signs and hemato-biochemical parameters at 7<sup>th</sup> day post treatment.
Marbofloxacin,Flunixin,Treatment,pneumonia,lambs
https://zvjz.journals.ekb.eg/article_28668.html
https://zvjz.journals.ekb.eg/article_28668_a31ade149eea7157ed6fc8d25ce7db8c.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Biochemical Studies on Rosemary and Licorice against Lead -Induced Oxidative Stress in Rats
244
256
EN
Mohamed A.
Hashem
Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
mhashem.vet@gmail.com
Amany A.
Abdallah
Clinical Pathology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Ibtisam G.
Eldeen
Animal Health Research Institute, Zagazig branch, Egypt
Mahmoud M.
Amer
Animal Health Research Institute, Zagazig branch, Egypt
10.21608/zvjz.2017.28669
The present study was carried out to explore the effect of rosemary and licorice alone or in combination with lead acetate on the biochemical parameters of adult male albino rats. A120 rats were equally divided into 6 groups. Group1 was kept as a control group (negative control). Groups 2 and 3 received aqueous extract of rosemary (100 mg/kg BW/day) and licorice (150 mg/kg BW/day) alone, respectively. Group 4 was given lead acetate (0.2%) alone; Group 5 was administrated lead acetate (0.2%) in combination with the aqueous extract of rosemary and Group 6 received lead acetate (0.2%) in combination with licorice. All treatments were given orally in drinking water for 6 weeks. Blood samples were collected at 2<sup>nd</sup>, 4<sup>th</sup> and 6<sup>th</sup> weeks post-treatment. Results showed that chronic lead toxicity caused a significant disturbance in liver and kidney functions, as well as serum antioxidants and immunoglobulins. However, rosemary or licorice extracts restored such changes. This study revealed that lead exposure induced severe oxidative stress and disturbed simultaneously the serum biochemical profile of albino male rats and rosemary or licorice administration improved these disturbances.
liver enzymes,rosemary,licorice,creatinine,catalase
https://zvjz.journals.ekb.eg/article_28669.html
https://zvjz.journals.ekb.eg/article_28669_32de482fedc8e42210b61e243ac5e193.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Ameliorative effect of Phoenix dactylifera on adverse effects of Linezolid in rats
257
266
EN
Elsayed A.
Abdel Aziz
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Sameh M.
El-Nabtity
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mahmoud A.A.
Said
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
ph.mahmoud.91@gmail.com
10.21608/zvjz.2017.29245
Linezolid is one of the oxazolidinone antibiotics that is used to treat methicillin resistant <em>staphylococcus aureus </em>(MRSA) & vancomycin resistant <em>Staphylococcus aureus</em>. This study aimed to investigate the adverse effects of linezolid on bone marrow and, kidneys and their counteraction by using <em>Phoenix</em><em> dactylifera </em>methanolic extract in rats. It was found that oral administration of linezolid (100 mg/kg body weight) for 14 successive days induced a significant decrease in hemoglobin content (7.88±0.18 g/L) on the first day post-treatment, significant increase in serum urea (59.75±0.85) and creatinine (1.89±0.04) on the 14<sup>th</sup> day post- treatment. The concurrent oral administration of <em>Phoenix</em><em> dactylifera</em> methanolic extract (1000 mg/kg body weight) and linezolid (100 mg/kg body weight) for the same period corrected the damaging effects of linezolid of the hemoglobin content, urea, creatinine of treated rats. It was concluded that methanolic extract of phoenix dactylifera clearly ameliorated these damaging effects of linezolid.
Phoenix dactylifera,Linezolid,urea,creatinine,brain and bone marrow
https://zvjz.journals.ekb.eg/article_29245.html
https://zvjz.journals.ekb.eg/article_29245_c765cbeface9837ab45c6ab3b42faa09.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Pharmacological Studies on Bleomycin as Cancer Therapy and Protective Effect of Aged Garlic
267
276
EN
Sabry M.A.
Abdel-Motal
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
Mohamed H.
Khairy
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
Ahmed A. S.
Mohamed
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
Magdy F.
Abou El-Fotoh
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
profdrmagdyfekry@yahoo.com
Shimaa A.
Abdel –Aziz
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
10.21608/zvjz.2017.29247
The pharmacological impacts of bleomycin as anticarcinogenic agents and the protective effect of aged garlic were done in current study. Eighty mature male albino mice were divided into 8 groups (each ten mice), group one was kept as a negative control, group two which was injected intraperoteneally with bleomycin. Group 3, which was given orally aged garlic, group 4 was inoculated with Ehrlich Ascites Carcinoma cells (EAC) , animals of group 5were administrated aged garlic and injected with EAC cells while group 6 was given simultaneously EAC cells and bleomycin I/P , group 7 was injected bleomycin I/P and administrated garlic 8 days later and group 8 took both bleomycin and garlic before inoculation of EAC cells by 8 days. Our results revealed that there were side effects of bleomycin on GSH, ROS, CAT, GPX and GR in the inoculated groups with EAC cells and confirmed by biochemical analysis. Aged garlic suppresses the progression of EAC cells and prevents their developmental effects
Bleomycin,cancer therapy,Protective Effect,Aged Garlic
https://zvjz.journals.ekb.eg/article_29247.html
https://zvjz.journals.ekb.eg/article_29247_e56863f33febe3e88086ef2afe493179.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Protective Effects of Vitamin E on Memantine Adverse Effects
277
288
EN
Gamal El-Din
Shams
Pharmacology Department, Faculty of Veterinary, Zagazig University, 44511, Egypt
Mohamed H.
Khairy
Pharmacology Department, Faculty of Veterinary, Zagazig University, 44511, Egypt
Mohammed A.
Saleh
Pharmacology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt
Samar A.
Antar
Pharmacology Department, Faculty of Pharmacy, Horus university
samarantar38@yahoo.com
10.21608/zvjz.2017.29248
This study to evaluate the protective effect of Vitamin E (1mg/kg, P.O. once daily) on Memantine adverse effects (0.36mg/kg, P.O. once daily) and its effect on liver, kidney, blood and antioxidant enzymes for three weeks. Then tissue and blood samples were collected on 1st, 2nd and 3rd weeks post-treatment to assess the protective effect of Vitamin E. Our results indicated that Vitamin E has hepato-nephro-protective and there was a decrease in the elevation of liver enzymes caused by Memantine like ALT, AST and ALP and kidney parameters like creatinine, urea and uric acid, and there was a normalize in all blood parameters which decrease by Memantine and finally there was a significant increase in antioxidant enzymes like CAT, SOD, GPX and a significant decrease in MDA, beside showing decrease in hepatic steatosis (arrowhead) and decrease in the apoptotic and necrotic cells mostly within the centrolobular caused by Memantine administration, as demonstrated by hepatic histopathology. Also, showing decrease in tubular basophilia, and decrease in interstitial nephritis (arrow indicated leukocytic infiltration), as showed by kidney histopathology. Therefore, Vitamin E should be taken with Memantine to decrease its side effects
Memantine,neuropsychiatric diseases,Vitamin E
https://zvjz.journals.ekb.eg/article_29248.html
https://zvjz.journals.ekb.eg/article_29248_834e1b75c52bdff4fe259ddc7660f481.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
The Protective Effect of Pomegranate Juice in Silver Nanoparticles Induced Hepatotoxicity in Mature Male Albino Mice
289
295
EN
Amira A.
Sallam
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
Mona M.
Ahmed
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
Ali H.
Abou Hadeed
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
Magdy F.
Abou El-Fotoh
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
profdrmagdyfekry@yahoo.com
10.21608/zvjz.2017.29249
Nano-silvers (AgNPs) are widely used in medical and consumer products thanks to their excellent antimicrobial and anti-carcinogenic effects. Sixty mature male albino mice were randomly distributed into six groups (ten per group). The first group was kept as negative control, the 2<sup>nd</sup> one was injected with silver nanoparticles 78 mg/kg BW I/P for 14 days, group 3 was given pomegranate (20 ml/kg BW) orally for 28 days, in the fourth group pomegranate was administrated for 14 days, followed by AgNPs for another 14 days. The fifth one AgNPs were I/P injected for 14 days, followed by pomegranate for another 14 days. The last group received AgNPs and pomegranate on the same time with the same doses and route of previous groups. The results revealed that pomegranate has a protective impact on AgNPs toxicity in all groups. These results were clarified the findings of AST, ALT, SOD, CAT, MDA and GPX. The protective and treatment effects of pomegranate in hepatotoxicity were evidenced by regeneration of hepatocyte and kupffur cells.
pomegranate juice,silver nanoparticles,Hepatotoxicity,male,Albino mice
https://zvjz.journals.ekb.eg/article_29249.html
https://zvjz.journals.ekb.eg/article_29249_42c66b91deb1d3fcf7687b2ba01501da.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Toxicological Effects on Silver Nanoparticles as Anticarcinogenic Agent and its Treatment with Curcumin
296
304
EN
Magdy F.
Abou El-Fotoh
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
Ali H.
Abou Hadeed
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
Esraa K.G.
Kotb
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt
10.21608/zvjz.2017.29250
In this study, the efficacy of silver nanoparticles (AgNPs) for treatment the Ehrlich Ascites Carcinoma cells (EAC) and trials to overcome its side effects by administration of the curcumin were carried out. Hundred and fifty mature male albino mice were divided into fifteen equal groups. Negative control animals were I.P injected with sterile normal saline, Positive control: oral dose curcumin daily an oral dose of (400 mg/Kg BW)<strong>,</strong> suspended in dist. H<sub>2</sub>O dosed, in the induced cancer control group: mice were inoculated with EAC through serial I/P of 2.5 x 10<sup>6</sup> EAC tumor cells 0.2 ml at 5-8 days intervals<strong>.</strong> Inject I.P. with different doses of silver nanoparticles<strong>,</strong> 100µ AgNPs+induced tumor, 200 µ of AgNPs + tumor induction, l00 µ AgNPs+ curcumin (400 mg/kg) + tumor induction, 200 µ AgNPs + curcumin (400 mg/kg) + tumor induction, 100 µ AgNPs before tumor induction by 15 days , 200 µ AgNPs buffer tumor induction 15 days then tumor induction EAC, 100 µ AgNPs + curcumin 400 µM/kg b.wt.) before induced tumor 15 days then induced tumor EAC, 200 µ AgNPs + curcumin 400 mg/kg bw.t) before induced tumor then tumor induction EAC, 100 µ AgNPs after tumor cell implantation 8 days later, 200 µ AgNPs after tumor cell implantation 8 days later, 100 µ AgNPs + curcumin 400 mg/kg BW) after tumor induction 8 day and 200 µ AgNPs + curcumin 400 mg/kg bw.t) after 8 days tumor induction. All results and histopathologyfindings validated that the silver nanoparticles are good for treatment of EAC and the curcumin can overcome their side effects.
silver nanoparticles,EAC Cancer Cells,Curcumin
https://zvjz.journals.ekb.eg/article_29250.html
https://zvjz.journals.ekb.eg/article_29250_33d3343a035c7165609259a9747458e2.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Virological and Immunological Diagnosis of Bovine Viral Diarrhea Virus in Rabbits
305
313
EN
Sherine M.
Zaghlal
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt/
Animal Reproduction Research Institute, Giza, Egypt
dr_chrry54@yahoo.com
Ali A.A.
Salama
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohammed EL-Bakry A.
Ismail
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Gamelat K.
Farag
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Manal B.
Mahmoud
Animal Reproduction Research Institute, Giza, Egypt.
Ahmed Abd El-Samie H.
Ali
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.29253
Bovine viral diarrhea (BVD) is a viral infectious disease of cattle. Other animals may act as reservoirs of BVDV; Rabbits are wide range in many countries, frequently existing close to farm animals. Recent studies revealed that BVDV propagated in rabbits through different routes of infections. Thus, New Zealand White rabbits were infected with two biotypes of BVDV (NADL and 890) through different routes (intravenous, nasal and oral). No clinical signs appeared on the infected rabbits. On the other hand, the lymphoid organs (GALF) of ileum of rabbits of BVDV infected groups and sacrificed at day five post infections showed depletion in the population of lymphocytes in the center of some lymphoid follicles. By RT-PCR some buffy coat samples and most organ samples in all exposed groups were virus positive at day five post infection, however samples of mock-infected control group remained negative. Antibodies development was observed in all groups infected animals, while in mock-infected control group, there was not production of antibodies. Innate immunity was detected by assessment of lysozyme and nitric oxide concentrations. There was an increase in lysozyme and nitric oxide concentrations in BVDV exposed groups when compared with mock infected group after day three as a defense mechanism of the host against the virus. Conclusion, these results indicates that rabbits are susceptible to infection by BVDV biotypes without clinical signs and lesions.
BVDV,Rabbit,GALF,PCR,lysozyme,Nitric oxide
https://zvjz.journals.ekb.eg/article_29253.html
https://zvjz.journals.ekb.eg/article_29253_7c4cc579e49f3ad9c1ea396abebc22e7.pdf
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
45
Supplementary 1
2017
10
01
Detection of Bovine Viral Diarrhea Virus in some Vaccines, Sera and Cell Cultures
314
322
EN
Eman M.
Hafz
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Ali A.A.
Salama
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Adel M.
Soliman
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Mohammed EL-Bakry A.
Ismail
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Gamelat K.
Farag
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
sendooo2002@gmail.com
Ahmed Abd El-Samie H.
Ali
Virology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
10.21608/zvjz.2017.29255
Bovine viral diarrhea virus (BVDV) causes contagious subclinical viral infections among dairy and beef cattle leading to economic losses worldwide. BVDV belongs to Family Flaviviridae, Genus Pestiviruses which is RNA viruses. It has two genotypes (I & II), each genotype has two biotypes, cytopathic (CP) and non-cytopathic (NCP) according to its cytopathology on cell culture. There is a risk factor from using bovine serum as a contaminant of the biological reagents and products prepared on cell cultures as vaccines. The study aimed to detect BVDV contamination in some biological agents such as (cell culture, serum and vaccines) using culture methods, ELISA and quantitate real time PCR. Samples consisted of 13 different attenuated and killed vaccines that are used in farm vaccination, five different serum samples that are used in cell culture and vaccines production in addition to three different cell culture samples that used in cell culture either for virus isolation or vaccines production. All samples inoculated on MDBK cells and examined for cytopathic BVDV revealed negative results. Cell culture and serum samples examined by ELISA technique also gave negative results. All samples were negative with real time PCR except one sample was positive. Comparing between all methods used, there was agreement between their results except in one sample that give positive result only by real time PCR. Therefore, we conclude that the chances of BVDV spread and contamination still there. The qRT – PCR is the most accurate method and can amplify a very little amount of virus. We also approve that all manufactured serum, vaccine samples were good as they were free from contamination with BVDV.
BVDV,PCR,ELISA,cell culture,serum,vaccine
https://zvjz.journals.ekb.eg/article_29255.html
https://zvjz.journals.ekb.eg/article_29255_ed0212b29579adeac08082e74dc04407.pdf