eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
1
10
10.21608/zvjz.2017.7681
7681
Original Article
Immunomodulatory Effects of Tulathromycin in Rabbits
Sabry Abdel-Motal
1
Gamal Shams
2
Nagah Edress
3
Abeer Anwer
4
Ghada Mohamed
ghada_sharkawy97@yahoo.com
5
Pharmacology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Pharmacology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Pharmacology Department, Faculty of Veterinary Medicine, Zagaig University, 44511, Egypt
Immunopharmacology Unit, Animal Reproduction Research Institute, Egypt
Directorate of Veterinary Medicine, Zagazig, Egypt
Tulathromycin is a new injectable macrolid antibiotic used for treating pulmonary disease. Therefore, the present work was aimed to study the effect of tulathromycin administration only or in a combination with vitamin C (immunomodatory agent) on immnomodulating, apoptotic effect and DNA of some immune cells. Moreover, to investigate its effect on serum antioxidant activity. Twenty-five apparently healthy rabbits were divided into 5 equal groups, the 1st group was the control and the 2nd was the vaccinated group with 1 mL/rabbit S.C “Pasteurella multocida vaccine”. The 3rd group injected with 17.5 mg/rabbit of vitamin C and 1 mL/rabbit S.C “Pasteurella multocida vaccine” while, the 4th group was given 1 mL S.C/rabbit Pasteurella multocida vaccine and 2.5 mg/kg BW tulathromycin. The 5th group was treated S.C with 17.5 mg/rabbit vitamin C, 1 mL Pasteurella multocida vaccine and 2.5 mg/kg BW tulathromycin. The results showed a significant inhibition of lymphocyte transformation at 3rd day, phagocytic activity and lysozyme activity at 1st, 2nd and 3rd day of vaccination in the 4th group. Moreover, its total globulin level was significantly depressed at the 7th and 14th day with a depression of antibody titre against pasteurella till the 3rd week post vaccination. Comet results revealed a significant increase of DNA damage % on the 3rd and 7th days post vaccination. DNA fragmentation of neutrophil was transiently occurred in the 3rd and 7th days post treatment. It was concluded that, Tulathromycin has a transient immunosuppressive and genotoxic effect, therefore it should be administered in a combination with Vit C to overcome its side effects.
https://zvjz.journals.ekb.eg/article_7681_bd2c1f29c1b879b0d8e59bbacf145ce3.pdf
Tulathromycin
vitamin c
immunity
genotoxicity
Rabbits
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
11
18
10.21608/zvjz.2017.7682
7682
Original Article
Isolation and Molecular Characterization of Infectious Bronchitis Virus from Broiler Chickens, Egypt during 2014 -2016
Mohammed Lebdah
1
Ahmed Hegazy
2
Mohammed Hassan
3
Mai Mohammed
mahmoudsamir527@gmail.com
4
Avian and Rabbit Medicine Department, Faculty of Veterniary Medicine University, Zagazig, 44511, Egypt
Avian and Rabbit Medicine Department, Faculty of Veterniary Medicine University, Zagazig, 44511, Egypt
Poultry Diseases Department, Animal Health Research Institute, P.O. Box 264-Dokki, Giza, 12618, Egypt
Poultry Diseases Department, Animal Health Research Institute, P.O. Box 264-Dokki, Giza, 12618, Egypt
In this study,surveillance on infectious bronchitis virus strains in 75 Egyptian broiler chicken flocks was performed using real-time RT-PCR at eight Governorates during 2014-2016. The birds in the selected flocks were suffering from respiratory and renal manifestations. The results of the present study showed that 31 (41%) samples were positive for IBV. All isolates were classified as variants using sequencing analysis of the obtained data and can be divided into 2 groups. Group I (variant) Egyptian IBV included isolates, EG-F728-1-2015-SP1, EG-F728-7-2015-SP1, EG-F862-3-2015-SP1, EG-F862-4-2015-SP1, EG-F888-2015-SP1, EG-F777-2015-SP1 and EG-F183-1-2016-SP1 which were very close to IBV-IS-1494-06 and had amino acid sequence identities between 87.5% to 96.2%. Group II (variant) Egyptian IBV included isolates, EG-F183-2-2016-SP1 and EG-F183-3-2016-SP1. These two IBV isolates were very close to IBV-IS/885 (variant 2) and they shared amino acid sequence identities from 92.3% to 97.1% with each other. In conclusion, different variants of IBV are present in broiler farms in high prevalence rate even in vaccinated flocks.
https://zvjz.journals.ekb.eg/article_7682_423a696479e3c41f2f53ec8b968a5fef.pdf
IBV
broiler
Egypt
Sequencing
Variants
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
19
28
10.21608/zvjz.2017.7683
7683
Original Article
The Effect of Carob Pods and Fig Fruits Ether Extracts against Lead Induced Hematological and Biochemical Changes in Oreochromis niloticus
Ahmed Said
1
Mohamed Kairy
2
Adel Shalaby
3
Hazem Ismail
hazemgreashclar@yahoo.com
4
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Fish Physiology Department, Central Laboratory for Aquaculture Research, Abbassa, Abo Hammad, Sharkia, Egypt
Fish Physiology Department, Central Laboratory for Aquaculture Research, Abbassa, Abo Hammad, Sharkia, Egypt
The current study aimed to investigate the possible effect of Ceratonia siliqua (carob) pods and Ficus carica (fig) fruits ether extracts against lead toxicity on hematological, biochemical parameters, growth performance and mortality % in Nile Tilapia (Oreochromis niloticus). One hundred and forty fish were divided into 7 groups. The first group was left as a control and fed a control diet, while, the second and third groups were exposed to 1/2 96 h LC50 (77.5 mg/L) and 1/4 96 hLC50 (38.7 mg/L) of Lead (Pb), respectively and provided with a control diet. The fourth and fifth groups were exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb, respectively and fed diet with 600 mg carob extract/kg diet. The sixth and seventh group were exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb, respectively and provided with diet contain 600 mg fig extract/kg diet. The hematological, biochemical parameters and growth performance was significantly decreased after exposure to 1/2 96 h LC50 and 1/4 96 hLC50 of Pb for 30, 60 and 90 days, while plasma glucose and mortality % were increased significantly. There were no significant changes in RBCs, Hb and PCV of O. niloticus exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb that fed diet enriched with carob extract 600 mg/kg diet for 90 days. The fish exposed to 1/2 96 h LC50 and 1/4 96 h LC50 of Pb and fed diet enriched with 600 mg/kg diet carob or fig for 90 days revealed no significant changes in plasma albumin, total protein, glucose and growth parameters with a reduction of mortality %. The current study highlighted the importance of carob and fig ether extract in the protection of Nile tilapia against the lead acetate toxicity.
https://zvjz.journals.ekb.eg/article_7683_cb1c1c6086d184a85a1f477c6bbaddb4.pdf
carob
Fig
Lead
Oreochromis niloticus
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
29
38
10.21608/zvjz.2017.7684
7684
Original Article
Molecular Detection of some Antimicrobial Resistance Genes in Salmonella Species Isolated from Commercial Layers in Egypt
Mohammed Lebdah
1
Waffa Mohammed
2
Samah Eid
3
Rehab Hamed
rehab.44444@yahoo.com
4
Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Reference Laboratory for quality control on poultry production (RLQP). Animal Health Research Institute, Dokki Lab., Giza, Egypt
Reference Laboratory for quality control on poultry production (RLQP). Animal Health Research Institute, Dokki Lab., Giza, Egypt
Reference Laboratory for quality control on poultry production (RLQP). Animal Health Research Institute, Sharkia Branch, Egypt
The present study was done to investigate the wide spread resistance to some antimicrobial groups among Salmonellae isolated from replacement and layer flocks in Egypt. A total of 24 salmonellae were isolated from 200 birds (apparently healthy or diseased suffered from diarrhea, dehydration, respiratory distress and decrease of egg production) and serotyped into S. Enteritidis, S. Typhimurium, S. Kentucky and S. Newport. Twenty-one Salmonella isolates were examined for resistance genes against different antimicrobials. The resistance pattern of all Salmonella isolates was done using antibiogram, the resistant isolates were examined for the presence of resistance coding genes using PCR technique. The investigated resistance genes were (qnrS, aac (6')-ib-cr) for quinolone resistant isolates, blaTEM for β-lactam resistant isolates, aadA1 and aadA2 for aminoglycosides resistant isolates and tetA(A) and tetA(B) for tetracycline resistant isolates. Resistant genes percentages for tetA(A), tetA(B), blaTEM, aadA1, aadA2, aac (6')-ib-cr and qnrS in the examined isolates were 70%, 20%, 93.3%, 30%, 80%, 10% and 15%, respectively. In conclusion, at the study area, antimicrobial resistance genes are widely spread in Salmonella isolates. Thus, minimizing the influence of antibiotics in treatment and prevention.
https://zvjz.journals.ekb.eg/article_7684_c9bf3550839d400f310beaf0ff833ddd.pdf
Salmonella
resistance genes
Replacement Layer
layers
Egypt
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
39
46
10.21608/zvjz.2017.7685
7685
Original Article
Determination of Marbofloxacin residues in Rabbit Tissues by HPLC
Elsayed Abdel Aziz
1
Sameh El-Nabtity
2
Abdel Azeem El Barawy
3
Mohammed Saleh
mohammedsaleh85_doctor@msn.com
4
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Animal Health Research Institute, Dokki, Giza, Egypt
Animal Health Research Institute, Dokki, Giza, Egypt
The present study was designed to evaluate marbofloxacin residues in different rabbit tissues after multiple intramuscular administrations. For that purpose, rabbits were divided into two groups; the first group (n=21) administered 2mg/kg marbofloxacin for five successive days, while the second group (n=3) were untreated and served as controls. Tissues were collected at the 1st, 3rd, 5th, 7th, 9th, 15th and 21stday after the last dose post administration of the drug. Liver, kidneys, pectoral muscle and thigh muscles, spleen, heart, blood and lung from each rabbit were taken, extracted and marbofloxacin residues were analyzed using high-performance liquid chromatographic method with ultraviolet detection. Results indicated a widespread distribution of marbofloxacin in the most tested tissues. It remained within detectable level till the 5th day in liver and serum while it continues till the 7th in kidneys day following the last dose. Therefore, muscles of rabbits treated with marbofloxacin could be consumed safely following the 1st day post treatment, while liver and kidneys could be consumed safely in the 3rd day after treatment without any hazards on consumers as the residual level below the recommended MRL (150µg/kg).
https://zvjz.journals.ekb.eg/article_7685_4a4dbc6c81e228f17a5eb9ccfb341007.pdf
Marbofloxacin
residues
Rabbits
HPLC
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
47
54
10.21608/zvjz.2017.7686
7686
Original Article
Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk
Waffa Ahmed
1
Amera Samer
amerashraf92@yahoo.com
2
Food Hygiene Department, Animal Health Research Institute, Zagazig Provincial Lab, Egypt
Microbiology Department, Animal Health Research Institute, Zagazig Provincial Lab, Egypt
One hundred random samples of raw buffalo and pasteurized cow milk (50, each) were collected from different localities of Sharkia Governorate for the detection of E. coli. The isolates were screened by PCR for virulence associated genes as well as antibiotic sensitivity test to determine the most effective antimicrobial agent. Furthermore, an experimental study was carried out to detect the influence of pasteurization and processing of kariesh cheese and yoghurt on E. coli O111:H8 inoculated in milk with the concentration of 108 CFU/mL. The obtained results showed that the occurrence of E. coli in the examined raw buffalo and pasteurized cow milk samples were 66% and 30%, respectively. Serogrouping of E. coli isolates revealed that O128, O26 and O111 were recorded as the most frequent O-serogroups. The sensitivity test showed that E. coli isolates were more sensitive to gentamicin (79.2%), followed by ciprofloxacin (70.8%) and colistin (68.8%). However, the examined isolates were completely resistant to erythromycin (100%) followed by sulphamethazole- trimethoprim (79.2%). Molecular identification of virulence associated genes revealed stx1, stx2 and eaeA genes. The experimental study showed that milk pasteurization was more effective on E. coli O111:H8 survival at refrigeration temperature compared with processed products such as kariesh cheese and yoghurt.
https://zvjz.journals.ekb.eg/article_7686_54b5d705297e8771fd72af94dbeec6e5.pdf
E. coli
Serotypes
stx1
stx2
eaeA
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
55
63
10.21608/zvjz.2017.7687
7687
Original Article
Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria
Adel Attia
1
Mohammed Hassan
2
Shymaa Elwan
shymoibrahim654@gmail.com
3
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Beta-lactamases (bls) are implicated seriously in resistance to β - lactam antibiotics. β- lactam resistance related to Sulfa hydral variabl- beta lactamases (SHV- bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained beta-lactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12.It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs.
https://zvjz.journals.ekb.eg/article_7687_9f2961c8921bd60bd71706ba9d4e506e.pdf
Extended spectrum beta lactamases
Sulfa hydral variable (SHV)
E. coli
Klebsiella pneumoniae
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
64
73
10.21608/zvjz.2017.7688
7688
Original Article
Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources
Adel Attia
1
Ahlam Gharib
2
Ibrahim Mohamed
3
Omnia Ahmed
o.ewasy@yahoo.com
4
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Food Hygiene Department, Animal Health Research Institute (AHRI ARC 60019332), Zagazig Provincial Lab
Microbiology Department, Animal Health Research Institute (AHRI ARC 60019332), Zagazig Provincial Lab
Enterococci are reservoirs for transmission of the most clinically important antimicrobial resistances such as vancomycinresistance. Therefore, this work aimed to determine the occurrence of enterococci and their respective vancomycine resistance genes (vanA and vanB) from different sources. Two hundred and twenty-four samples from chickens, turkey, fish and human urine, as well as, two types of human food including milk (raw and milk from mastitic animals) and sausage were tested for isolation of Enterococcus species. The isolates wereidentified morphologically and biochemically usingcatalase test, sodium chloride tolerance and growth at pH 9.6and 10- 45˚C. The vancomycin resistance profile of the isolates was verified by both disc diffusion and agar dilution methods. The genotypic enterococcal identification at both genus and species levels and their vancomycine resistance genes were also ascertained using PCR amplification of the respective genes for 28 isolates. Enterococci isolation rate was 70% of the examined samples with a higher percentage of vancomycine resistance (53.5%) and the minimum inhibitory concentrations (MICs) ranged from 16 to 512 µg/mL. Molecular identification of 28 enterococcal isolates revealed the dominance of E. faecalis (42.8%) and clarified a higher proportion of vanA (78.5%) and vanB (67.8%) genes. In conclusion, administration of the antimicrobials mainly vancomycin may be considered as a pronounced stress factor in the veterinary and human practices. In addition, VRE can act as a reservoir for vancomycin resistance.
https://zvjz.journals.ekb.eg/article_7688_b961571c733a223ba0da760853fecee0.pdf
Antimicrobial susceptibility
Vancomycin resistance
enterococci
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
74
81
10.21608/zvjz.2017.7689
7689
Original Article
Hormonal and Immunological responses to Coleus forskohlii treatment in Female Rats with Experimentally Polycystic Ovaries Syndrome
Hoda Abd-Allah
hoda72001@yahoo.com
1
Mona AbdAlhady
2
Magda Abdelall
3
Sahar Mohamedy
4
Waheeb Alharbi
5
Adil Bahathiq
6
Animal Health Research Institute, Zagazig Laboratory, Egypt
Physiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Animal Health Research Institute, Zagazig Laboratory, Egypt
Animal Health Research Institute, Zagazig Laboratory, Egypt
Physiology Department, Faculty of Medicine, Umm al Qura University, Makkah, KSA
Physiology Department, Faculty of Medicine, Umm al Qura University, Makkah, KSA
Polycystic ovaries syndrome (PCOS) is one of the most prevalent causes of reproductive failure. Its pathogenesis may be hormonal and immune disorder with hyperactivity of the sympathetic nervous system. The present study was conducted to demonstrate the effect of coleus forskohlii roots extract (CFE) in modulating the levels of some hormones and immune parameters of rats with androgen induced PCOS. Fifty immature female albino rats were divided into control group and androgen treated group that injected subcutaneously with 60 mg/kg BW/day androgen (Cidotestone) for 35 days to induce PCOS. Each group was subdivided into two subgroups. The first two subgroups received saline and 25 mg/kg BW of CFE, respectively while the other two subgroups received the same doses of Cidotestone and Cidotestone + CFE, respectively for 3 weeks. Blood samples were collected at the end of the experiment to estimate the levels of corticosterone, beta endorphin, total leucocytic count (TLC), cluster of differentiation 4 (CD+4), cluster of differentiation 8 (CD+8), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), immunoglobulin G (IgG) and immunoglobulin A (IgA). The results revealed a significant decrease in the concentrations of β endorphin, IL-2, TLC, CD+4, CD+8 and IgG, while corticosterone and IL-4 showed a significant increase in the androgen induced PCOS group. Rats treated with Cidotestone + CFE displayed normal values of all tested parameters except corticosterone, suggesting role of CFE in mediating both humoral and cellular immunity.
https://zvjz.journals.ekb.eg/article_7689_9328d3089ec1b95be598ec450e3c67ee.pdf
Coleus forskohlii
Rats
Polycystic Ovaries Syndrome
eng
Zagazig University, Faculty of Veterinary Medicine
Zagazig Veterinary Journal
1110-1458
2357-075X
2017-03-01
45
1
82
91
10.21608/zvjz.2017.7690
7690
Original Article
Presynchronization with CIDR improved the efficiency of ovsynch in the cyclic and acyclic Postpartum Pluriparous Cows
Ahmed Ahmed
ezzat_29@yahoo.com
1
Alaa Eldin Zain Elabdeen
2
Omar Zain
3
Theriogenology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
Theriogenology Department, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt
Theriogenology Department, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt
The postpartum period is considered an important period in the reproductive life of dairy cows.This study was aimed to evaluate the effect of progesterone (P4)-CIDR supplementation in the course of Ovsynch regimens in postpartum Holstein-Friesian dairy cows. Eighty Holstein Friesian dairy cows were used. All animals were gynaecologically examined for uterine involution and ovarian resumption by rectal ultrasound scan. Those with history of endometritis, caesarean section, dystocia, retained placenta, acute mastitis, lameness or other unhealthy were excluded. Fifty-two cows were cyclic and twenty eights were non-cyclic. Animals were divided into three synch-regimens. The 1st group (N=27), used as a control, is the ovsynch regimen only in which the animals were treated with GnRH-PGF2α-GnRH (GPG), the 2nd group (N=26) in which the GPG was presynchronized with controlled internal drug release (CIDR) device before (CIDR-GPG), and the 3rd group (N=27) in which CIDR device was incorporated into GPG, after the first GnRH injection (G-CIDR-PG). Blood samples were collected 5 times (day -7, 0, 7, 14 and 50) for measuring P4 using enzyme-immunoassay (EIA) technique. Ultrasound examinations were performed on days -7, 0, 7 and 9 for following up the ovarian changes, and repeated at 35, 45 and 50 days post-insemination for pregnancy diagnosis. Conception rate was variable among groups. It was increased by 69.23, 51.85 and 40.74% in CIDR-GPG, G-CIDR-PG regimens treated groups and GPG-control group, respectively. The conception rate was higher in the cyclic versus acyclic GPG group (44.4 vs. 33.3 %). On the other hand, acyclic cows were highly responsive than cyclic cows in CIDR-GPG group (70.0 vs. 68.8%) and G-CIDR-PG group (55.6 vs. 50.0%). In conclusion, we recommend that the CIDR-GPG regimen is improving fertility and conception rate in postpartum dairy cows.
https://zvjz.journals.ekb.eg/article_7690_2bb738cc0247ad516bd7131f229512cb.pdf
CIDR
cow
conception
Ovsynch