@article { author = {Basset, Aly AE and Seleem, AA and Mohamed, Sh Kh}, title = {Plastination of Plexuses Brachialis, Lumbosacralis And Cauda Equina Using In Education Of Neuroanatomy Of The Goat}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {1-12}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59956}, abstract = {Plastination of nervous tissue has become a very important tool in teaching neuroanatomy. Also, plastinated nervous specimens can be used to study the anatomical relations in clinics right before surgical operations. The aim of using plastinated models is to increase knowledge, understanding as well as imagination of the complex anatomical parts of the central nervous system. The plastinated anatomical teaching model of Medulla spinalis, Plexus brachialis, Plexus lumbosacralis and Cauda equina of goat displays a clear morphology that corresponds qualitatively to the actual cadaver specimens. Apparently healthy four goats (ten months age) from native breed were used. Goats were subjected to silicone S10 plastination technique. Prior to plastination, the goat was prepared by I/V injection of Xylazine Hcl (0.01mg/kg), cutting Arteria carotis communis for complete bleeding and injected via the latter artery with 10% formalized solution then immersed in the same solution for at least four weeks. Medulla spinalis and attached peripheral nerves, Plexus brachialis, Plexus lumbosacral and Cauda equina were dissected. The present study provided an enhancement for teaching neuroanatomy of the goat, especially Medulla spinalis, Nervi cervicales, Plexus brachialis, Nervi thoracici, Plexus lumbosacralis and Cauda equina. Understanding the anatomy of the nervous system is important not only for veterinary students but also for veterinary doctors while undertaking surgery on that plexuses and nerves.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59956.html}, eprint = {https://zvjz.journals.ekb.eg/article_59956_b5b38260a49ccf431032eba5c3d00ed5.pdf} } @article { author = {Hakim, Y and Hassanin, M El-Sayed and Abdallah Ali, Haytham}, title = {Chronic Effect of Fenitrothion on Health of Oreochromis niloticus and Oxidative Stress Biomarkers}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {13-22}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59957}, abstract = {Serious problems of pollution and health hazards accompanied the wide range of production and application of pesticides during the last few years had occurred. Ninety Oreochromis niloticus were exposed to 1/10 and 1/20 96 hrs LC50 to assess its chronic injurious effect on growth performance, biochemical analysis and histopathological alteration. The results revealed that a significant decrease in the final body weight, weight gain and body gain percentage and condition factor. The usual inhibition of Acetylcholine esterase (AChE) was detected that result in the detected behavioral changes. There was significant increase in superoxide dismutase enzyme activity (SOD), malondialdehyde (MDA), alanine aminotransferase (ALT), creatinine and urea levels with significant reduction in serum glutathione (GSH) level  and total serum Immunoglobulon M (IgM). The detected histopathological alterations in liver, kidney and gills were related to the concentration and duration of exposure to Fenitrothion.}, keywords = {Fenitrothion insecticide,Oreochromis niloticus,health and growth,Oxidative Stress,prooxidant activity}, url = {https://zvjz.journals.ekb.eg/article_59957.html}, eprint = {https://zvjz.journals.ekb.eg/article_59957_e33b0ce252e28e8c319dc78573eede97.pdf} } @article { author = {HH, Allam and Salah ABS, Aly and R H, Zaki and A Bahar, Nagwa}, title = {Bacteriological and Biochemical Studies On Pekin Duckling Infected With Pasteurella Multocida with Trial For Treatment}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {23-32}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59958}, abstract = {Samples from cloacal swabs, liver, heart, lungs, trachea, spleen and nasal exudate were collected from 150 pekin duckling ageing 1-30 days (75 apparentaly healthy 35 diseased and 40 freshly dead) for bacteriological examination. Out of 150 examined sample 43 (28.67 %) were positive for Pasteurella Multocidanamely 5 from apparentally healthy, 13 from diseased and 25 from dead ducklings. Antibiogram study of isolates revealed that florfenicol was the highly effective drug against Pasteurella Multocida. A total of 160, one day old pekin duckling (80 healthy & 80 experimantally infected with Pasteurella Multocida) were divided into 4 equal groups (40 birds each), the 1st group consisted of healthy ducklings (control), the 2nd group included healthy ducklings which were treated with florfenicol (30mg/kg BW) in drinking water for 5 days, while the 3rd group was infected non treated ducklings and the 4th group was infected and treated ducklings with florfenicol (same dose, period and route of administration). In the four groups the hemato-biochemical changes were studied. The results revealed that healthy pekin ducklings treated with florfenicol displayed significant rise in body weight, leukocytic count and lymphocytosis, with significant decrease in heterophils. There was insignificant increase in monocytes, eosinophils, basophils, total proteins, albumin, globulins, A/G ratio, AST, ALT, ALP, uric acid and creatinine. Pasteurellosis in duckling induced anorexia depression, ruffled feathers, coughing, diarrhea, rise respiratory rate and 30% mortality rate. Monocytes, total, β, γ globulin, AST, ALP, uric acid, creatinine levels and significant decrease in weight gain, heterophils, albumin, α globulin, A/G ratio, insignificant decrease in leukocyte, lymphocyts, esinophils, total proteins and insignificant rise ALT.[M1]  Florofenicol residues in examined liver and kidneys in both treated healthy and diseased ducklings were high at 1st day post treatment, very low at 6th day and completely negative from examined samples at 9th day post treatment. The highest levels of florofenicol residues were recorded in kidneys then liver. Duckling suffering from pasteurellosis and treated with florfenicol showed no clinical signs, mortality rate 5%, reduced re-isolation of Pasteurella Multocida and improved hemato-biochemical parameters. It could be concluded that florfenicol is effective in treating Pasteurella Multocida in duckling.  }, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59958.html}, eprint = {https://zvjz.journals.ekb.eg/article_59958_27783d5e622a6abe9d7bc69d6fcc85d0.pdf} } @article { author = {A Mohammed, Haiam and E Abd el-Hamid, Nora and MMZ, Hamada and AA, El-Badry}, title = {Vitamin D And Male Reproduction}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {33-40}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59959}, abstract = {To study the relationship between vitamin D and male reproduction, fifty weanlings (21 days-old) male albino rats were obtained and divided into 2 groups, vitamin D-deficient group fed vitamin D-deficient diet and vitamin D-replete group fed the same diet but received a single intraperitoneal injection of 2 µg cholecalciferol per week (0.1 ml propylene glycol). The animals in both groups were maintained till became adult. The results revealed detection and expression of vitamin D receptor (VDR) gene in the testis of young rats at 2 months of age, a significant increase in acrosome reaction percentage, sperm motility and sperm count in the vitamin D-replete group, while sperm abnormalities were significantly higher in the vitamin D-deficient group. The results of this study suggest an important physiological role played by vitamin D in male reproductive biology. }, keywords = {Vitamin D,Reproduction,Male rat}, url = {https://zvjz.journals.ekb.eg/article_59959.html}, eprint = {https://zvjz.journals.ekb.eg/article_59959_309980f5c34c3489d8b90ddc3243da8b.pdf} } @article { author = {El Sayed El Sayed Ahmed, Sabry and Abdel Razek, Mohga and El Sherbeny Ramadan, Mahmoud and Esmail Mohamed Esmail, Eman}, title = {Hepatic Coccidiosis In Rabbits And Comparative Study On Treatment With Herbal Drug}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {41-50}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59960}, abstract = {Twenty five Rabbits were collected and showed, Eimeria stiedae Oocyst from both fecal and liver smears (40%) when examined microscopically. Naturally infected rabbits showed emaciation, diarrhea and yellowish white colour nodules on the liver. Forty rabbits of two months age were divided into 4 groups; one kept as control, the last 3 groups were infected with the isolated oocyst and each rabbit received 100000 sporulated oocyst per 3ml. The orally infected group only showed severe signs as diarrhea, decrease in body weight, loss of appetite and 30% mortality. Moreover, damaged and enlarged liver with hepatomegally, yellowish white nodules on the surface and severe histopathological lesions in liver and bile duct. Group treated with 4% Neem extract  (Herbal anticoccidial) showed normal body weight which increased post treatment and improved the liver function (AST and ALT), while group treated with Toltrazuril showed a significant increase in body weight more than the control group. Also, liver function improvement was more responded with no symptoms in both treated groups.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59960.html}, eprint = {https://zvjz.journals.ekb.eg/article_59960_2c1fcb70729e80575ebc5585fa4eaf1d.pdf} } @article { author = {M. El–Bayomi, Khairy and S. El-Tarabany, Mahmoud and A.F. Nasr, Mohammed and M. Roushdy, El-Shimaa}, title = {Effects of Divergent Selection for Growth on Carcass Traits in Japanese Quails}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {51-56}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59961}, abstract = {Japanese quail has been used as a model for studying the genetics of growth-selected poultry and poultry breeding. The aim of this study was to determine the effects of selection for growth on carcass traits in different lines of Japanese quails. Three lines of Japanese quails were selected (300 birds aged 2 weeks) from the original population including high body weight (HBW), low body weight (LBW) and random bred control (RBC). At 4th week of age, birds were weighed, slaughtered, weighed the carcass, edible giblets and ratio of these organs to the empty body weight were recorded. After the third generation of selection, the selected high line showed significantly higher (p < 0.05) slaughter, dressing and carcass weights compared to selected low and control lines. The mean carcass weights were 90.95 g in control, 56.12 g in low and 127.05 g in high line. These weights represented approximately 0.76, 0.62 and 0.78 of live body weight at 4th week of age, respectively. Liver, gizzard, spleen and heart weights were significantly increased (P < 0.05) in selected high line when compared to low and control lines. The mean of liver, gizzard, spleen and heart weight in selected high line were 5.85, 5.96, 0.40 and 2.54 g, which represented about 0.04, 0.04, 0.002 and 0.02 of live body weight at 4th week of age, respectively.  It concluded that selection for high body weight has been improved the carcass traits, including yield and quality.}, keywords = {selection,Growth,carcass,Japanese quails}, url = {https://zvjz.journals.ekb.eg/article_59961.html}, eprint = {https://zvjz.journals.ekb.eg/article_59961_800a9cf30dc4c5b155f712400810438a.pdf} } @article { author = {Zaid, Rehab and Shehata, Youssef and Dowidar, Mohamed and Abdallah Ali, Haytham and Mohamed Abdelazim, Aaser}, title = {Biochemical Effect Of L-Carnitine Against Doxorubicin And Vancomycin Induced Lipid Disorders In Rats}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {57-67}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59962}, abstract = {The ameliorative effect of L-carnitine on the lipid, liver and kidneys disorders induced by doxorubicin and vancomycin was examined. Ninety male albino rats were randomly divided into six groups (15) each; control, L-carnitine treated, Doxorubicin treated, vancomycin treated, doxorubicin + L-carnitine, Vancomycin + L-carnitine. Animals were sacrificed after 3, 10, 15 days. Blood and tissue samples were collected. Leptin, insulin, MDA, HDL, TC, TP and albumin levels, LDH and GGT activities were determined. mRNA expression levels of leptin, leptin receptors and GAPDH genes were determined in adipose tissues. There was a significant increase of serum insulin, leptin, MDA, HDL, TC levels, GGT and LDH activities and significant decrease of Albumin, TP levels in the groups treated with doxorubicin and Vancomycin. In conclusion, DOX and VAN had a bad effect on the lipid profile as they induced obesity. Also they increased the liver enzymes, heart markers and the oxidative stress in body tissues due to their ability to increase the level of MDA. L-carnitine administration ameliorated the hazards effect of DOX and VAN on the lipid sate in the body.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59962.html}, eprint = {https://zvjz.journals.ekb.eg/article_59962_e5fb51fc3592ba711159354d53562500.pdf} } @article { author = {F E, Shaaban and A H, Abo Hadeed and G Moustafa, Gihan and M Elhady, Walaa}, title = {Genotoxic Studies Of Roundup And Stomp Herbicides On Nile Catfish (Clarias Gariepinus)}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {68-80}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59963}, abstract = {The present study was designed to evaluate the genotoxic effects of Roundup, Stomp herbicides and their combination on Nile catfish (Clarias gariepinus). The experiment was carried out on 120 fish that randomly divided into four equal groups with three replicates. The first group kept as a control, the second group exposed to 1/2 96 hrs LC50 of roundup, the third group exposed to 1/2 96 hrs LC50 of Stomp and the fourth one exposed to a combination of roundup and Stomp with the previously mentioned doses. The experiment was terminated at 15 days after blood samples collection and then the sera were separated for estimating 8-hydroxy 2-deoxy guanisine (8-OHdG) then the fish were sacrificed and two specimens from gills of all groups were obtained and kept at -20ºC for comet assay and the other specimens were fixed in 10% neutral –buffered formalin for histopathological examination. The results indicated that both herbicides individually cause significant increase in DNA damage that was highly obvious in the group exposed to combination of both herbicides. Histopathological investigation of gills confirmed the aforementioned findings.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59963.html}, eprint = {https://zvjz.journals.ekb.eg/article_59963_246eb49e1eb269031dc1c237659ed172.pdf} } @article { author = {MY, Abdel-Hamed and AA, Abou-Zaid and IM, Eisa and M Fakhry, Hiam and M I El-Neshwy, Wafaa}, title = {Application Of Recent Methods For Diagnosis Of Foot And Mouth Disease In Cattle And Buffaloes}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {81-90}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59964}, abstract = {Egypt was recently exposed to a sever outbreak of Foot and Mouth Disease (FMD) in 2012 with a new introduced strain of the virus (SAT2). This study concerned with diagnosis of FMD by using some recent methods in addition to the traditional methods of diagnosis. A total of 326 animals were clinically examined in different localities at Sharkia Governorates; 301 animals showed clinical signs of FMD (diseased animals) and 25 animals were apparently healthy. The diseased animals consisted of 277 cattle and 24 buffaloes. Some of them were vaccinated with the FMD bivalent vaccine before the outbreak and others were not vaccinated. Eighty two  clinical samples (73 epithelial tissues (Epi.T.) and 9 vesicular fluids (V.F)) from cattle and buffaloes were isolated on Baby Hamster kidney (BHK-21) cell line and 76 samples (92.7%) were positive. By using FMDV ELISA Kits for typing the virus only serotype SAT2 was detected. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect SAT2 serotype in 10 sample collected from 5 diseased cattle and 5 diseased buffaloes from different localities. Only one sample showed negative reaction (this sample was also negative by virus isolation and ELISA typing). Serum samples were collected from the 301 animals at different times after the infection; the antibodies against FMDV non-structural protein were detected by FMDV′CHEK Test in 270 cases (89.7%) of examined animals, while antibodies against the structural protein were detected by SNT and ELISA where antibodies against serotypes O, A and SAT2 were detected in 75.7%, 75.7% and 100% of examined animals for each serotype respectively by both tests. Mixed infection with more than one serotype was only recorded at Al-Salhia dairy farm. It is concluded that combination between recent and traditional techniques for diagnosis of FMD help in accurate and rabid diagnosis and subsequently control the disease.}, keywords = {FMD,Diagnosis,ELISA kits,RT-PCR,FMDV′CHECH Test}, url = {https://zvjz.journals.ekb.eg/article_59964.html}, eprint = {https://zvjz.journals.ekb.eg/article_59964_ae8c9b858d3c70fe2fff894a720dcbf4.pdf} } @article { author = {A Abd El Ghany, Nadia and S Elias, Nashwa}, title = {A Risk Assessment Of Fungal Infection With Aspergillus flavus In Oreochromis niloticus Through A Laboratory-Acquired Infection}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {91-103}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59965}, abstract = {In the current research, Oreochromis niloticus fish were subjected to experimental infection by Aspergillus flavus and treated by the garlic powder (immunostimulants) in the feed. The infected fish exhibited signs of protrusive eye, slow-motion, lethargy, skin covered by thick mucus, severe hemorrhage on dorsal, anal and caudal fins, discarded scales and caudal fin erosion. Postmortem examination revealed that accumulative of bloody fluids in viscera and the abdominal cavity, damaged liver and enlargement of gall bladder which was filled with bile. Treatment trials exerted zero mortality with Allium sativum (garlic) but group treated by Aspergillus flavus with Allium sativum the mortality rate was 20% while the mortality rate was 100% with Aspergillus flavus group . Hematological picture showed normocytic normochromic anemia in both Aspergillus flavus and Aspergillus flavus with Allium sativum treated groups. Leucopenia and lymphopenia observed in Aspergillus flavus group, while lymphocytosis was reported in Allium sativum treatment group. There were significant increase in liver transaminases enzymes (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) and decrease in total protein and albumin level in Aspergillus flavus but in Aspergillus flavus with Allium sativum significantly reversed the biochemical changes in the blood serum AST, ALT , total protein and albumin level. Histopathological findings of gills in fish group that was infected with Aspergillus flavus were severe in forms of proliferation of the filamentary epithelium leading to lamellar fusion of the secondary lamellae and desquamation, particularly at the tips and the pathological changes in the liver showed severe blood congestion and hemolytic with clear diffusion of melanomacrophage (MMC), thrombosis in blood vessels and severe congestion in pancreatic acini. These lesions may become less severe in fish of Aspergillus flavus with Allium sativum group. It could be concluded that the safety and useful dietary addition of Allium sativum (garlic) to alleviate the effects of Aspergillus flavus on Oreochromis niloticus fish.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59965.html}, eprint = {https://zvjz.journals.ekb.eg/article_59965_851eb2ca32432537b79351ec0eb97a74.pdf} } @article { author = {M, Taha and SI, Essa and AI, Younes}, title = {Phenotyping And Genotyping Identification Of Important Human, Animal And Soil Of Dermatophytes}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {104-123}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59966}, abstract = {In the current study, 62 dermatophyte isolates obtained from human and animal dermatophytosis as well as from soil were subjected for phenotypic and genotypic identification. The conventional (phenotypic) method for identification of dermatophytes in the present work was succeeding to identify all isolates through macro-morphology, micro-morphology and differential media into species. Identification of 48 isolates obtained from human cases revealed 15 M.canis, 12 T. violaceum, 12 T. rubrum, 5 E. fluccosum and 4 T. mentagrophytes. The eight isolates obtained from cattle were identified as T. verrucosum, while the four isolates obtained from dogs and cats were identified as M. canis. On the other hand, two dermatophyte isolates obtained from soil were identified as M. gypseum. On the other hand, three methods were used for molecular (genotypic) identification of dermatophytes which include: a) PCR for amplification of ITS1 and ITS4 followed by restriction fragment length polymorphism (RFLP) using MvaI for 30 isolates of dermatophyte formerly identified by phenotypic method, b) Application of PCR using single repetitive oligonucleatede (GACA) 4 for 30 dermatophyte isolates formerly identified by phenotypic and RFLP methods, c) DNA sequencing which done  for 5 representative isolates of M.canis, T.verrucosum, T.violaceum and T.rubrum. While, RFLP using Mval method and repetitive(GACA) 4 method identified the 30 dermatophyte isolates into species identical to those identified by phenotypic methods, sequencing identified one isolate formerly identified by phenotypic, MvaI and (GACA) as T. rubrum with similarity 99% as T. raubitschekii. Although molecular methods are rapid and represents technological advance in the laboratory diagnosis, it is expensive. So, we recommended its use in absence of skilled mycologist in identification of atypical or variants of dermatophyte species.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59966.html}, eprint = {https://zvjz.journals.ekb.eg/article_59966_281b6a25f15acf9ea5ca2401cec65028.pdf} } @article { author = {M M, Taha}, title = {Isolation and Identification of Clostridium difficile from Horses}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {124-130}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59967}, abstract = {Clostridium difficile is considered one of the most important causes of enterocolitis associated with diarrhea in horses especially foals that may lead to necrohaemorrhagic enterocolitis with high mortality rate. Diarrhea mostly developed while treating with antibiotics, however, it may develop without any history of antibiotics treatment. In this study, twenty faecal samples and seven rectal swabs from foals suffering from diarrhea were collected and cultured onto Cefoxitin Fructose Agar (CFA) as specific growth medium. Isolates from twenty-one (77.7%) of these samples gave the characteristic microscopical examination and colony morphology as well as they gave negative results with catalase and oxidase tests. Also, they gave fluorescence after exposure to long wave length ultraviolet light. Specific agglutination kit was used for confirmation, revealed nineteen isolates (70.4%) gave positive for agglutination. The A and B crude toxins were prepared from the isolates, toxogencity test in mice was done for the prepared toxins as preliminary test for toxogencity.Nine (33.3%) isolates found to be lethal to mice, then SDS-PAGE was done to the fifth isolate (as it gave the highest MLD, indicating its the most toxogenic isolate) where the toxins gave bands at molecular masses of 308 and 270 kDa, which  resemble that of toxins A and B of Clostridium difficile, respectively. Finally, the isolated Clostridium difficile recommended to be used in preparation of vaccine for controlling the severity of enterocolitis disease among horses.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59967.html}, eprint = {https://zvjz.journals.ekb.eg/article_59967_a8b0498d7a90ca8859789e98aa0b2137.pdf} } @article { author = {M S, Nada and A I I, Badawy and M I A, Mona}, title = {Assessment of Sarcocystis fusiformis, Whole Cyst Extract Antigen from Buffaloes in Diagnosis of Cattle Sarcocystosis}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {131-136}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59968}, abstract = {In this study, the whole extract antigen of Sarcocystis fusiformis from buffaloes was assessed for diagnosis of cattle sarcocystosis using ELISA. Prevalence rate of Sarcocystis species among cattle using microscopical examination was 87.69%, while the prevalence rate was 92.31 % by using ELISA. The sensitivity and specificity of ELISA in diagnosis cattle infection were 93.33% and 80%, respectively. We strongly recommended the use of combined microscopical examination and ELISA test for Sarcocystis diagnosis, due to the presence of hidden or microscopic cysts, to avoid human infection of such zoonotic parasite and to control the consequent disease. This study concluded that the use of whole cystic crude antigen of S. fusiformis derived from buffaloes is a reliable mean for diagnosing cattle sarcocystosis}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59968.html}, eprint = {https://zvjz.journals.ekb.eg/article_59968_3f9dfceff019d37500c6c7cc83febd4d.pdf} } @article { author = {Ali AbuShaala, Faraj and Mustafa Lapez, Rima}, title = {Evaluation of the Efficacy of Hepatitis B Vaccine for Children In Misurata Region, Libya}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {137-144}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59969}, abstract = {Hepatitis B virus (HBV) is a blood borne and sexually transmitted virus. Rates of new infection and acute disease are high among adults, but chronic infection is more likely to occur in persons infected as infants or young children aged 2-5 years old. Hepatitis B vaccination is the most effective measure to prevent HBV infection and its consequences. This study was aimed to evaluate the effectiveness of HBV vaccine in randomly selected children from Misurata province, Libya and personal factors associated with serologic evidence of the immune response.  Serum samples for each participant were tested for the quantitative determination of anti-HBs antibody using Enzyme Linked Fluorescent Assay. The results of antibody response to hepatitis-B vaccine among children cases included in the current study indicated that antibody decay occurred with time. In our study negative antibody response were observed in 21% and 17% among male and female children, respectively whom were vaccinated according to the official vaccination schedule and their ages range was 2-5 years old. These negative percentages were increased to 27% and 29% in male and female children, respectively whom age range was 6-8 years old. While the percentages were 33% and 25% in male and female children, respectively of age ranged from 9-12 years old. Antibody prevalence titer was higher in older male and female children than younger children as the antibody titers were 33, 25; 27,29; and 21, 17 respectively.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59969.html}, eprint = {https://zvjz.journals.ekb.eg/article_59969_4a9f9cb4360a509afcdab651228394cf.pdf} } @article { author = {A Basset, Aly El-Deen and A Mohamed, Hassan and M EA Omar, Ahmed and E A Ahmed, Enas}, title = {The Plastinated Abdominal Muscles of the Goat As A Future Trend In Teaching Anatomy}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {145-152}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59970}, abstract = {The application of plastination technique as a new resource in anatomical education of abdominal muscles is of importance in the era of dwindling of cadavers for Veterinary Medical Education in Egypt due to the high cost of animals. Raising the advocacy for using plastinates as adjunct to cadavers is needed to complement the learning and teaching of Veterinary anatomy. The objective of this research was to describe the method of developing plastinated specimens of the goat abdominal muscles for educational purposes. A better understanding of abdominal muscles anatomy is relevant to gynecology, surgery and internal medicine. We recommend that the use of plastinated specimens is an excellent method for teaching abdominal muscles of goat.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59970.html}, eprint = {https://zvjz.journals.ekb.eg/article_59970_b351986dd52423eec7fc88983bb4665d.pdf} } @article { author = {M Ibrahim, Fatma and A Mansour, Hassan and M Labib, Faten and A Amer, Hussein and R Beheiry, Rasha}, title = {Morphological Studies in Cryopreserved Buffalo Bull Sperm}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {153-169}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59971}, abstract = {The present work was designed to study the ultrastructure of buffalo bull spermatozoa and to recognize the possible defects that may occurre following the cryopreservation process. Fresh semen samples were collected from three buffalo-bulls from Mahallet Musa buffalo research station in Kafr El-shiekh Governorate for production of buffalo-bull sires. Straws of cryopreserved semen were obtained from Kafr El-Shiekh Center for frozen buffalo-bull semen. All these samples were prepared for examination by transmission electron microscope. The results revealed that the freshly collected spermatozoa from apparently healthy buffalo bull consisted of head and tail. The tail attached to the head axially and consisted of neck, middle piece, principle piece and end piece. The head consisted of the acrosome and the nucleus surrounded by the plasma membrane. The neck located between the head and middle piece and had fossa in which the centriole was located. The middle piece was characterized by the mitochondrial sheath that surrounded the flagellar core in 9+2 axonemic arrangement and the nine outer dense fibers (ODF). The principle piece was the longest portion of the spermatozoon. It had flagellar core, dense fibers and fibrous sheath. The fibrous sheaths were made of two longitudinal columns, a dorsal and a ventral one, and connecting ribs. The end piece contained only the axial filament complex. The results revealed the effect of cryopreservation on ultrastructure of buffalo bull spermatozoa; disruption, undulation and damage of plasma membrane, detached and disintegration of acrosome, decondensation of chromatin of the nucleus, bent and swelling middle piece, breakage of the plasma membrane of middle piece and loss of some of the mitochondria, irregular shaped mitochondria, irregular arrangement and shape of dense fibers and distorded axonemes.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59971.html}, eprint = {https://zvjz.journals.ekb.eg/article_59971_c22a799014127efffe851fc2aba39418.pdf} } @article { author = {A Aly, Mohamed and M S Ammar, Saied and A Mohamed, Hassan and A A Mahdy, Eman}, title = {Some Morphological Studies on Prostata (Prostate Gland) of Buffalo Bull (Bos Bubalis L.)}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {170-180}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59972}, abstract = {The objective of this research was to describetheanatomical and histological structure of the prostate gland in buffalo bulls. This study was carried out on the prostate gland of 25 animals. The gland consisted of two parts; Corpus prostatae and Pars disseminate prostatae. The shape of the former part was variable (band like, two lobed connected by an isthmus (Isthmus prostatae) and three lobed gland). It was situated transversally on the dorsal aspect of the pelvic urethra just caudal to the dorsal fibrous cord of the vesicular gland. Pars disseminata prostatae occupied in the entire length of the pelvic urethra. Microscopically, the capsuleof the gland was fiberomuscular in nature. Fiberomuscular septa arose from the capsule dividing the gland into lobes. The latter septa gave rise of fiberoelastic septulae, which dividing the lobes into lobules. The prostatic secretory end-pieces were of ubule-alveolar type, which were lined with simple cuboidal epithelium. These cells reacted positively with PAS and alcian blue stains. The intralobular ducts were lined with simple cuboidal epithelium, changed into simple columnar in the interlobular ducts and transitional type in the main excretory ducts, at their opening in the pelvic urethra.}, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59972.html}, eprint = {https://zvjz.journals.ekb.eg/article_59972_9c3d4bfa78c841f6e5e1fbc8f034f27c.pdf} } @article { author = {AM, Ammar and EM, Riad and M Heba, Rihan and BA, Asmaa}, title = {Bacteriological Studies on the Effect of Some Disinfectants and Antibacterial Agents on Mycobacterium tuberculosis Complex Isolated from Human and Animal Samples}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {181-188}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59973}, abstract = {This study was carried out to evaluate the effect of some disinfectants and antibacterial agents on Mycobacterium tuberculosis complex isolated from animal and human samples. The animal samples were collected from suspected animals from El-Sharkia, El- Menofya and El -Gharbiya governorate included lymph nodes (57 samples), raw milk samples (1250 sample) and human sputum samples (143) collected from the workers (attendance and cow-men) at infected farms. The bacteriological findings of the examined infected lymph nodes samples revealed that 31 sample out of 57 were harbored mycobacterium species with a percent of 54.4%, while 30 isolates of mycobacterium spp. were recovered on Lowenstein- Jenseen media with a percentage of 2.4% from the milk samples. On the other hand, the results of bacteriological examination of human sputum samples proved that 6 samples (4.2%) were harbored the acid fast bacilli by microscopical examination using Ziehl- Neelsen stain. The efficacy of both first and second line antituberculous drugs on the isolated strains were examined using method of proportion and showed that Rifampicin and Ethambutol had relative potent activity against the isolated mycobacteria also, Ciprofloxacin and Kanamycin had relative potent activity against the isolated mycobacteria. Evaluating the efficacy of disinfectants (phenol 5%-10% and ethyl alcohol 70%) using qualitative suspension test revealed that phenol 5% was effective at 20min exposure , phenol 10% was effective at 15min exposure and ethyl alcohol 70%  was effective at 20min.  }, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59973.html}, eprint = {https://zvjz.journals.ekb.eg/article_59973_d8a66e1ab3d4c5ee054dc796837870c5.pdf} } @article { author = {M A F, Doaa and S A M, Sydat and M F, Medhat and F D, Mohamed and A Ali, Haytham}, title = {Molecular, Biological and Biochemical Studies on Obesity in Relation to Antibiotics}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {189-196}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59974}, abstract = {This study was designed to investigate the correlation between obesity and antibiotics. The obesity was induced by high fat diet (corn oil diet) for 8 weeks. 60 male albino rats were divided into obese group (30 rats), and another non-obese group (30 rats), which subdivided into three groups. Group I (N=10) Control, Group II: (N=10) non obese-rats were received vancomycin 30mg/kg BW for 7days and Group III: (N=10) rats were received doxyrubicin 0.6mg/kg BW for 3 successive weeks and some rats were decapitated after each injection. Group IV (N=10) control obese. Group V (N=10) obese rats were received vancomycin with the same dose as in non obese group. Group VI (N=10) obese rats were received the same dose as in non-obese group. Body weight, leptin, lipid profile, lipid %, blood glucose, malondialdhyde and (HOMA-IR) hemostasis model assessment insulin resistance in the obese group injected I/P with vancomycin HOMA-IR in comparison to control group was increased. The level of serum leptin, insulin concentration in the obese group injected I/P with vancomycin was decreased, while the expression level of ob gene increased which investigate leptin resistance. Thus, the present study suggested relationship between antibiotics and obesity.}, keywords = {leptin,HOMA,Vancomycin,Doxorubicin}, url = {https://zvjz.journals.ekb.eg/article_59974.html}, eprint = {} } @article { author = {AM, Azab and A, Abd El-Kareem and Abo El-Yazed, Manal and SE, Mahdy}, title = {Correlation between FMDV and Antibodies in Milk and Blood of Infected Lactating Ewes}, journal = {Zagazig Veterinary Journal}, volume = {42}, number = {2}, pages = {197-209}, year = {2014}, publisher = {Zagazig University, Faculty of Veterinary Medicine}, issn = {1110-1458}, eissn = {2357-075X}, doi = {10.21608/zvjz.2014.59975}, abstract = {FMDV type O, A and their specific antibodies were detected in milk and blood of 2 groups of experimentally infected ewes aiming to investigate thecorrelation between FMDV and antibodies in milk and blood of infected lactating ewes. FMD virus types O and A inoculated into the mammary gland of dairy ewes one week post parturition. ELISA and polymerase chain reaction (PCR) were used as diagnostic tools for detecting FMDV in milk and blood from infected ewes. FMDV disseminated from the inoculated udder was firstly detected at 18th hours post inoculation using ELISA and (RT-PCR). FMDV were detected by ELISA with 80% for types O&A and 40& 60% for type O & A, respectively by tissue culture isolation. FMDV continued detected in milk for 23 days post infection in the two groups. Also FMDV (O &A) were detected in blood by ELISA at the 30th hours and continued for 8 days post infection. Specific protective antibodies were detected in blood and milk at the 2nd and 3rd weeks post udder inoculation (WPI), respectively by ELISA and the antibody titer continued with a protective level till 10 WPI in milk against type O&A. Seven from positive samples to FMD virus by ELISA were confirmed by RT-PCR, (four samples were serotype O and three were serotype A). The features that influence sample quality appear by RT-PCR as they can detect FMDV genomic RNA.Otherwise FMD antibody titer continued with a protective level till the 20th (WPI) in serum of infected ewes with type O & A. So, it is clear that there is a clear correlation between FMDV and its antibodies in milk and blood of infected ewes. These findings reflect the relationship between the incidence of FMDV and its antibodies in the milk and blood of infected ewes and spot the light on the possibility of FMDV transmission from ewes to their lambs through suckling and we paying attention to the importance of milk examination to detect the virus incidence as early as possible to avoid the outbreaks. }, keywords = {}, url = {https://zvjz.journals.ekb.eg/article_59975.html}, eprint = {} }